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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Yuan, Hongmei | - |
dc.contributor.author | Wu, Hongge | - |
dc.contributor.author | Cheng, Jing | - |
dc.contributor.author | Xiong, Jie | - |
dc.date.accessioned | 2023-02-03T10:37:19Z | - |
dc.date.available | 2023-02-03T10:37:19Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Histology and Histopathology Vol. 36, nº12 (2021) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/128146 | - |
dc.description.abstract | Background. The involvement of circular RNAs (circRNAs) in the development of cancers has attracted much interest. This study aimed to determine the role of circ_0000376 in non-small cell lung cancer (NSCLC) and provide a new mechanism. Methods. The expression of circ_0000376, miR488-3p and bromodomain containing 4 (BRD4) mRNA was measured by quantitative real-time PCR (qPCR). Cell behaviors, including cell proliferation, invasion, migration, apoptosis and cell cycle progression were investigated using cell counting kit-8 (CCK-8) assay and colony formation assay, transwell assay, wound healing assay and flow cytometry assay, respectively. The putative relationship between miR-488-3p and circ_0000376 or BRD4 was verified by dual-luciferase reporter assay. The protein levels of BRD4 and phosphorylated PI3K/PKB were detected by western blot. Xenograft model was constructed to determine the role of circ_0000376 in vivo. Results. Circ_0000376 was highly expressed in NSCLC tissues and cells. Circ_0000376 downregulation inhibited NSCLC cell proliferation, invasion and migration, promoted cell apoptosis and cell cycle arrest and slowed tumor growth in vivo. Circ_0000376 competitively bound to miR-488-3p to regulate the expression of BRD4. Rescue experiments showed that miR-488-3p deficiency reversed the effects of circ_0000376 downregulation, and miR-488-3p restoration-suppressed cell proliferation, migration and invasion were recovered by BRD4 overexpression. Moreover, circ_0000376 downregulation weakened the levels of phosphorylated PI3K and PKB, thus reducing the activity of the PI3K/PKB pathway. Conclusion. Circ_0000376 downregulation blocked the development of NSCLC by targeting the miR-488- 3p/BRD4 network and suppressing the PI3K/PKB pathway, which broadens knowledge into the understanding of the role of circ_0000376 in NSCLC. | es |
dc.format | application/pdf | es |
dc.format.extent | 16 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | circ_0000376 | es |
dc.subject | miR-488-3p | es |
dc.subject | BRD4 | es |
dc.subject | NSCLC | es |
dc.subject | PI3K/PKB | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Circ_0000376 downregulation inhibits the progression of non-small cell lung cancer by mediating the miR-488-3p/BRD4 axis and the PI3K/PKB signaling pathway | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-390 | - |
Aparece en las colecciones: | Vol.36,nº12 (2021) |
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Yuan-36-1309-1324-2021.pdf | 19,85 MB | Adobe PDF | Visualizar/Abrir |
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