Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.14670/HH-18-324
Twittear
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Lopez-Trujillo, Emilio | - |
dc.contributor.author | Gonzàlez-Farré, Mònica | - |
dc.contributor.author | Pujol, Ramon M. | - |
dc.contributor.author | Bellosillo, Beatriz | - |
dc.contributor.author | Fisa, Roser | - |
dc.contributor.author | Riera, Cristina | - |
dc.contributor.author | Alcover, Magdalena | - |
dc.contributor.author | Barranco, Carlos | - |
dc.contributor.author | Martin-Ezquerra, Gemma | - |
dc.date.accessioned | 2023-01-13T10:40:46Z | - |
dc.date.available | 2023-01-13T10:40:46Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Histology and Histopathology Vol. 36, nº5 (2021) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/127284 | - |
dc.description.abstract | Background. Different immunohistochemical markers to detect amastigotes in cutaneous Leishmaniasis have been proposed with variable diagnostic usefulness. Objectives. To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous Leishmaniasis. Materials and methods. Thirty-three skin samples corresponding to PCR confirmed cutaneous Leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results. From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, antiCD1a and anti-Leishmania, was 94% [CI 95%: (79,8%- 99,3%)]; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. identification. Conclusions. Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin – eosin and Giemsa staining. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Amastigote | es |
dc.subject | CD1a | es |
dc.subject | Immunohistochemistry | es |
dc.subject | Leishmania | es |
dc.subject | Leishmaniasis | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal antiLeishmania antibodies in cutaneous Leishmaniasis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-324 | - |
Aparece en las colecciones: | Vol.36, nº5 (2021) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Lopez-Trujillo-36-567-576-2021.pdf | 22,85 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons