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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Liu, Ya-Kun | - |
dc.contributor.author | Jia, Ya-Jing | - |
dc.contributor.author | Liu, Shi-Hao | - |
dc.contributor.author | Shi, Hong-Jie | - |
dc.contributor.author | Ma, Jing | - |
dc.date.accessioned | 2023-01-12T10:13:53Z | - |
dc.date.available | 2023-01-12T10:13:53Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Histology and Histopathology Vol. 36, nº5 (2021) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/127191 | - |
dc.description.abstract | Objective. To investigate the effect of the downregulation of FXYD domain-containing ion transport regulator 5 (FXYD5) on the cisplatin resistance (CisR) of epithelial ovarian cancer (EOC) cells. Methods. A2780-CisR and SKOV3-CisR cells were obtained through repeated administrations of different cisplatin concentrations, and the half-maximal inhibition concentration (IC50) was calculated by MTT assays. After transfection with FXYD5 siRNA-1 and FXYD5 siRNA-2, the IC50 values of the A2780-CisR and SKOV3-CisR cells were also detected by the MTT method. Cell proliferation, migration, invasion and apoptosis were evaluated through 5-ethynyl-2'- deoxyuridine (EdU) DNA synthesis, wound healing, Transwell invasion and Annexin-V-FITC/PI dualstaining assays, respectively. qRT-PCR and Western blotting were conducted to detect mRNA and protein expression. Results. Compared with the sensitive parental cells, the A2780-CisR and SKOV3-CisR cells had increased IC50 and FXYD5 expression. FXYD5 siRNA reduced the IC50 value of cisplatin in the A2780-CisR and SKOV3-CisR cells and decreased the expression of ABCG2 (BCRP) and ABCB1 (MDR1). In addition, FXYD5 inhibition reduced the invasion and migration of the A2780-CisR and SKOV3-CisR cells, with upregulation of E-cadherin and downregulation of Snail and Vimentin. Both FXYD5 siRNA-1 and FXYD5 siRNA-2 inhibited the proliferation and promoted the apoptosis of the A2780-CisR and SKOV3-CisR cells with reduced Ki-67 and increased caspase-3. Conclusion. FXYD5 downregulation may reduce the invasion, migration and EMT formation of EOC cells to increase their sensitivity to cisplatin chemotherapy by inhibiting cell proliferation and promoting cell apoptosis. | es |
dc.format | application/pdf | es |
dc.format.extent | 11 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Epithelial ovarian cancer | es |
dc.subject | FXYD5 | es |
dc.subject | Cisplatin | es |
dc.subject | Drug resistance | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Low expression of FXYD5 reverses the cisplatin resistance of epithelial ovarian cancer cells | - |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-310 | - |
Aparece en las colecciones: | Vol.36, nº5 (2021) |
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Liu-36-535-545-2021.pdf | 32,82 MB | Adobe PDF | Visualizar/Abrir |
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