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https://doi.org/10.14670/HH-18-298
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Título: | Propofol protects PC12 cells from cobalt chloride-induced injury by mediating miR-134 |
Fecha de publicación: | 2021 |
Editorial: | Universidad de Murcia, Departamento de Biologia Celular e Histiologia |
Cita bibliográfica: | Histology and Histopathology Vol. 36, nº4 (2021) |
ISSN: | 0213-3911 1699-5848 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Propofol MicroRNA-134 PC12 Cobalt chloride |
Resumen: | Objective. Propofol (PRO) was reported to exert a neuroprotective effect by decreasing microRNA134 (miR-134), a brain-specific miRNA, thus, the role of PRO against cobalt chloride (CoCl 2)-induced injury in rat pheochromocytoma cells (PC12) via mediating miR134 was explored. Methods. CoCl 2-induced PC12 cells treated with PRO were transfected with or without miR-134 negative control (NC)/ inhibitor/mimic, and the following detections were then performed using cell counting kit-8 (CCK-8), Annexin V-fluorescein isothiocyanate/ propidium iodide (Annexin V-FITC/PI) and Hoechst 33258 staining. Autophagy was observed by transmission electron microscope (TEM). Mitochondrial membrane potential (MMP) was detected by Rhodamine-123 (Rh123) staining, and reactive oxygen species (ROS) by dichloro-dihydro-fluorescein diacetate (DCFH-DA) staining. Protein and gene expressions were measured by Western blotting and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), respectively. Results. PRO reversed the CoCl 2-induced decrease in the PC12 cell viability and increased miR-134 in a dose-dependent manner. CoCl 2 increased LC3II/I ratio and Beclin-1 expression, but decreased p62 expression, which was abolished by PRO. In addition, an increased cell apoptosis rates triggered by CoCl 2 were reduced by PRO with the down-regulations of Bax and Caspase-3 and the up-regulation of Bcl-2. Furthermore, PRO decreased methylenedioxyamphetamine (MDA), nitric oxide (NO) and ROS in CoCl 2-induced PC12 cells accompanying the increase in glutathione peroxidase (GSH-Px) and MMP. The effects of PRO on autophagy, apoptosis and oxidative stress in CoCl 2-induced PC12 cell were reversed by miR-134 mimic. Conclusion. PRO may mitigate CoCl2-induced autophagy in PC12 cells with decreased apoptosis and improved oxidative stress via mediating miR-134. |
Autor/es principal/es: | Zhou, Hong-Yi Jiang, Fan Cao, Zhong Shen, Qi-Yun Feng, Yu-Jing Hou, Zhen-Huan |
URI: | http://hdl.handle.net/10201/127144 |
DOI: | https://doi.org/10.14670/HH-18-298 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 11 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.36, nº4 (2021) |
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Fichero | Descripción | Tamaño | Formato | |
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Zhou-36-425-435-2021.pdf | 19,5 MB | Adobe PDF | Visualizar/Abrir |
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