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https://doi.org/10.14670/HH-18-276
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Título: | Transplantation of mesenchymal stem cells preserves podocyte homeostasis through modulation of parietal epithelial cell activation in adriamycin-induced mouse kidney injury model |
Fecha de publicación: | 2020 |
Editorial: | Universidad de Murcia, Departamento de Biologia Celular e Histiologia |
Cita bibliográfica: | Histology and Histopathology Vol. 35, nº12 (2020) |
ISSN: | 0213-3911 1699-5848 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Mesenchymal stem cells Parietal epithelial cells Podocytes Profibrotic cells Acute kidney injury Chronic kidney disease |
Resumen: | To determine the role of the transplantation of bone marrow-derived mesenchymal stem cells (MSCs) in podocyte renewal, we studied BALB/C mice with or without adriamycin-induced acute kidney injury. MSCs were transplanted ectopically under the capsule of the left kidney or into the peritoneal cavity after the onset of kidney injury to test their local or systemic paracrine effects, respectively. Adriamycin produced increases in urine protein: creatinine ratios, blood urea nitrogen, and blood pressure, which improved after both renal subcapsular and intraperitoneal MSCs transplants. The histological changes of adriamycin kidney changes regressed in both kidneys and in only the ipsilateral kidney after intraperitoneal or renal subcapsular transplants indicating that the benefits of transplanted MSCs were related to the extent of paracrine factor distribution. Analysis of kidney tissues for p57-positive podocytes showed that MSC transplants restored adriamycin-induced decreases in the abundance of these cells to normal levels, although after renal subcapsular transplants these changes did not extend to contralateral kidneys. Moreover, adriamycin caused inflammatory activation of PECs with coexpression of CD44 and phospho-ERK, which was normalized in both or only ipsilateral kidneys depending on whether MSCs were transplanted in the peritoneal cavity or subcapsular space, respectively |
Autor/es principal/es: | Aslam, Rukhsana Hussain, Ali Cheng, Kang Kumar, Vinod Malhotra, Ashwani Gupta, Sanjeev Singhal, Pravin C. |
URI: | http://hdl.handle.net/10201/126712 |
DOI: | https://doi.org/10.14670/HH-18-276 |
Tipo de documento: | info:eu-repo/semantics/annotation |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.35,nº12 (2020) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Aslam-35-1483-1492-2020.pdf | 12 MB | Adobe PDF | Visualizar/Abrir |
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