Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.14670/HH-18-196
Twittear
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Kim, Namyul | - |
dc.contributor.author | Choi, Kyeong Uk | - |
dc.contributor.author | Lee, Eunbee | - |
dc.contributor.author | Lee, Seoyun | - |
dc.contributor.author | Oh, Jiwon | - |
dc.contributor.author | Kim, Woo keyoung | - |
dc.contributor.author | Woo, Sang-Ho | - |
dc.contributor.author | Kim, Dae-Yong | - |
dc.contributor.author | Kim, Wan-Hee | - |
dc.contributor.author | Kweon, Oh-kyeong | - |
dc.date.accessioned | 2022-11-29T10:07:02Z | - |
dc.date.available | 2022-11-29T10:07:02Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Histology and Histopathology Vol. 35, nº7 (2020) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/126052 | - |
dc.description.abstract | Adipose-derived mesenchymal stromal cells (Ad-MSCs) have excellent potential for skin wound repair. Moreover, platelet-derived growth factor (PDGF) has strong wound healing properties. The purpose of the present study was to compare the healing effects of PDGF-overexpressing canine allogeneic Ad-MSCs (PDGF-MSCs) and their cell sheets (PDGF-CSs) as compared to unexpressed Ad-MSCs (U-MSCs) and their cell sheets (UCSs) in a cutaneous wound healing model induced upon dogs. In in vitro study, the expression of immunomodulatory and growth factors was assessed by qRT-PCR. In in vivo study, cells and sheets were transplanted into a square-shaped full-thickness (1.5×1.5 cm) skin defect model created in 12 dogs. After 5 and 10 days, wounds were harvested and evaluated macroscopically and histopathologically. The qRT-PCR results showed that the PDGF-B gene was significantly upregulated (p<0.05) in PDGF-CS and PDGF-MSCs groups. Upon gross analysis of the wound, all stromal cells and their sheet groups showed accelerated (p<0.05) cutaneous wound healing compared to the negative control groups. As compared to U-MSCs and UCSs, the PDGF-MSCs showed significant epithelization on days 5 and 10 of healing, whereas PDGF-CSs showed improved epithelization only on day 10. In the granulation tissue analysis, PDGF-CSs and UCSs promoted more formation (p<0.05) of upper granulation tissue, collagen, and activated fibroblasts than PDGF- MSCs, and U-MSCs. Especially, the PDGF-CSs presented the highest formation and maturation of granulation tissue among all groups. All considered, PDGF overexpressed stromal cells or cells sheets can improve cutaneous wound healing in a canine model. | es |
dc.format | application/pdf | es |
dc.format.extent | 17 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Platelet-derived growth factor | es |
dc.subject | Adipose- derivedmesenchymal stromal cell sheets | es |
dc.subject | Wound healing | es |
dc.subject | Dog | es |
dc.title | Therapeutic effects of platelet derived growth factor overexpressed-mesenchymal stromal cells and sheets in canine skin wound healing model | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-196 | - |
Aparece en las colecciones: | Vol.35, nº7 (2020) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Kim-35-751-767-2020.pdf | 29,81 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons