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dc.contributor.authorLeiva Cepas, Fernando-
dc.contributor.authorJimena, Ignacio-
dc.contributor.authorRuz Caracuel, Ignacio-
dc.contributor.authorLuque, Evelio-
dc.contributor.authorVillalba, Rafael-
dc.contributor.authorPeña Amaro, Jose-
dc.identifier.citationHistology and Histopathology Vol. 35, nº5 (2020)es
dc.description.abstractThe purpose of this study was to determine the histological characteristics of a skeletal muscle reconstructed by means of the implantation of autologous adipose tissue following an experimentally- induced volumetric muscle loss. A cylindrical piece in the belly of the rat anterior tibial muscle was removed. In the hole, inguinal subcutaneous adipose tissue of the same rat was grafted. Animals were sacrificed 7, 14, 21, 28 and 60 days posttransplantation. Histological, histochemical, immunohistochemical and morphometric techniques were used. At all times analyzed, the regenerative muscle fibers formed from the edges of the muscle tissue showed histological, histochemical and immunohistochemical differences in comparison with the control group. These differences are related to delays in the maturation process and are related to problems in reinnervation and disorientation of muscle fibers. The stains for MyoD and desmin showed that some myoblasts and myotubes seem to derive from the transplanted adipose tissue. After 60 days, the transplant area was 20% occupied by fibrosis and by 80% skeletal muscle. However, the neo-muscle was chaotically organized showing muscle fiber disorientation and centronucleated fibers with irregular shape and size. Our results support the hypothesis that, at least from a morphological point of view, autologous adipose tissue transplantation favors reconstruction following a volumetric loss of skeletal muscle by combining the inherent regenerative response of the organ itself and the myogenic differentiation of the stem cells present in the adipose tissue. However, in our study, the formed neo- muscle exhibited histological differences in comparison with the normal skeletal musclees
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectSkeletal musclees
dc.subjectMuscle regenerationes
dc.subjectAdipose tissuees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleHistology of skeletal muscle reconstructed by means of the implantation of autologous adipose tissue: an experimental studyes
Aparece en las colecciones:Vol.35, nº5 (2020)

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