Por favor, use este identificador para citar o enlazar este ítem: https://doi.org/10.14670/HH-18-117

Título: Differential severity of LPS-induced lung injury in CD26/DPP4 positive and deficient F344 rats
Fecha de publicación: 2019
Cita bibliográfica: Histology and Histopathology Vol. 34, nº 10 (2019)
ISSN: 0213-3911
1699-5848
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología
Palabras clave: Collectins
CD26/DPP4
Ultrastructure
Inflammation
LPS
Resumen: Background. Lipopolysaccharide (LPS) induced inflammation often leads to lung injury, in which pulmonary recruitment of neutrophils plays a pivotal role. Inflammatory processes are influenced by CD26/DPP4, highly expressed in lungs. Asthma induced CD26/DPP4 deficient (CD26/DPP4 - ) Fischer (F) 344 rats suffering from a transport block in the rER caused by a point mutation showed reduced pulmonary inflammation and reduced expression of immuno- modulating surfactant proteins (SP). The degree of LPS induced lung injury in CD26/DPP4 deficient rats has not been investigated so far. Objective. We hypothesize that LPS induced lung injury leads not only to an attenuated inflammation but also to a reduced SP expression and decreased structural damage in CD26/DPP4 - rats. Methods. Both genotypes were intratracheally instilled with 250 μl LPS or with 250 μl 0.9% NaCl. Nine hours later animals were killed and either bronchoalveolar lavage was carried out to determine inflammatory cells and surface tension or lung blocks were removed and processed for histology, immunohistochemistry, electron microscopy or qRt-PCR analyses and Western Blot analyses. Results. Signs of acute lung injury, such as structural damage of the blood gas barrier occurred only sporadically in both genotypes. LPS-induced CD26/DPP4 - rats showed decreased gene expression of SP-A and SP-D and reduced signs of lung inflammation associated with a reduced alveolar influx of macrophages and neutrophils. Conclusions. Less pulmonary inflammation combined with less structural alterations and minor expression of immunomodulating SP may be an indication of the critical role of CD26/DPP4 in regulating lung inflammation
Autor/es principal/es: Zientara, Alicja
Stephan, Michael
von Hörsten, Stephan
Schmied, Andreas
URI: http://hdl.handle.net/10201/124686
DOI: https://doi.org/10.14670/HH-18-117
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 21
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Vol.34,nº10 (2019)

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