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DOI: 10.14670/HH-18-107
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Otsuki, Shuhei | - |
dc.contributor.author | Alvarez Garcia, Oscar | - |
dc.contributor.author | Lotz, Martin K. | - |
dc.contributor.author | Neo, Masashi | - |
dc.date.accessioned | 2022-07-11T07:29:20Z | - |
dc.date.available | 2022-07-11T07:29:20Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Histology and Histopathology, Vol.34, nº9, (2019) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/122264 | - |
dc.description.abstract | The expression of heparan sulfate endosulfatases (Sulfs) was investigated in the intervertebral disc (IVD) to clarify their role in IVD homeostasis. Sulf-1 and -2 expression were elucidated in normal and degenerated human IVD. Age-related effects on Sulf expression, type II collagen levels, and structural changes were analyzed in IVDs of wild-type (WT) and Sulf-1 knockout (Sulf-1-/- ) mice. The effect of recombinant Sulf-1 (100 ng/ml) and Sulf-1 knockdown on heparan sulfate proteoglycan and collagen expression in ATDC5 cells were examined. Finally, the effect of Sulf-1 on transforming growth factor (TGF) β1-induced signaling was evaluated. Results show that Sulf-1 and -2 levels were higher in degenerated human IVDs. In WT mice, Sulf-1 and -2 expression generally declined as the animals aged. In particular, Sulf-1 in the nucleus pulposus was higher compared with Sulf-2 at the age of 1 and 6 months and significantly declined with aging. Sulf-1-/- mice showed more severe IVD pathology than WT mice, with lower type II collagen levels in nucleus pulposus, and degeneration with type I collagen in annulus fibrosus. In vitro, Sulf-1 induced type II collagen expression and significantly increased TGF-β1- induced Smad2/3 phosphorylation in ATDC5 cells. In conclusion, Sulf-1 might play a critical role from development to maintenance of IVD homeostasis by regulating collagen expression. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Intervertebral disc | es |
dc.subject | Sulf-1 | es |
dc.subject | Type II collagen | es |
dc.subject | Smad2/3 | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Role of heparan sulfate 6-0 endosulfatases in intervertebral disc homeostasis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | DOI: 10.14670/HH-18-107 | - |
Aparece en las colecciones: | Vol.34, nº9 (2019) |
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Otsuki-34-1051-1060-2019.pdf | 17,53 MB | Adobe PDF | Visualizar/Abrir |
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