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DOI: 10.14670/HH-18-059
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Título: | A plant proteinase inhibitor from Enterolobium contortisiliquum attenuates airway hyperresponsiveness, inflammation and remodeling in a mouse model of asthma |
Fecha de publicación: | 2019 |
Editorial: | Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and Histopathology, Vol.34, nº5, (2019) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Asthma Pulmonary inflammation Plant proteinase inhibitor Enterolobium contortisiliquum Trypsin Inhibitor |
Resumen: | Introduction. Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for asthma. Purpose. The aim of the present study was to evaluate the effects of Enterolobium contortisiliquum trypsin inhibitor (EcTI) on pulmonary mechanical function, eosinophilic recruitment, inflammatory cytokines, remodeling and oxidative stress in an experimental model of chronic allergic pulmonary inflammation. Methods. BALB/c mice were divided into 4 groups: C (saline i.p and inhalations with saline), OVA (ovalbumin i.p and inhalations with ovalbumin); C+EC (saline i.p, inhalations with s aline and treatment with EcTI); OVA+EC (ovalbumin i.p, inhalations with ovalbumin and treatment with EcTI). On day 29, we performed the following tests: resistance (Rrs) and elastance (Ers) of the respiratory system; (b) quantify eosinophils, 8-ISO-PGF2α, collagen and elastic fiber volume fractions; (c) IFN-γ, IL-4, IL-5, IL-13, MMP-9, TIMP-1, TGF-β, iNOS and p65-NFκB-positive cells in the airway and alveolar walls. Results. In OVA+EC group, there was an attenuation of the Rrs and Ers, reduction of eosinophils, IL-4, IL-5, IL-13, IFN-γ, iNOS and p65-NF κB-positive cells compared to OVA group. The 8-ISO-PGF2α, elastic and collagen fibers volume fractions as well as the positive cells for MMP-9, TIMP1 and TGF-β positive cells were decreased in OVA+EC compared to the OVA group. Conclusion. EcTI attenuates bronchial hyperresponsiveness, inflammation, remodeling and oxidative stress activation in this experimental mouse model of asthma. |
Autor/es principal/es: | Dias Rodrigues, Adriana Palmeira Santos Bortolozzo, Anelize Sartori Arantes Costa, Fernanda Magalhães Saraiva Romanholo, Beatriz Mangueira Castro Ribas de Souza, Flávia Brüggemann, Thayse Regina Roncon Santana, Fernanda Paula Vilela de Brito, Marlon Ramalho Bonturi, Camila Neto dos Santos Nunes, Natalia Máximo Prado, Carla Leick, Edna Aparecida Vilela Oliva, Maria Luiza de Arruda Martins, Milton Fraga Righetti, Renato Lopes Calvo Tibério, Iolanda de Fátima |
URI: | http://hdl.handle.net/10201/121645 |
DOI: | DOI: 10.14670/HH-18-059 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 16 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.34, nº5 (2019) |
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Fichero | Descripción | Tamaño | Formato | |
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Rodrigues-34-537-552-2019.pdf | 12,1 MB | Adobe PDF | Visualizar/Abrir |
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