Por favor, use este identificador para citar o enlazar este ítem: DOI: 10.14670/HH-18-057

Título: Umbilical cord-derived mesenchymal stromal/stem cells enhance recovery of surgically induced skeletal muscle ischemia in a rat model
Fecha de publicación: 2019
Editorial: Universidad de Murcia. Departamento de Biología Celular e Histología
Cita bibliográfica: Histology and Histopathology, Vol.34, nº5, (2019)
ISSN: 1699-5848
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología
Palabras clave: Umbilical cord-derived mesenchymal stromal/stem cells
Allogeneic transplantation
Muscle ischemia
M2 macrophage activation
Resumen: This study delves into possible mechanisms underlying the stimulating influence of UC-MSCs transplantation on functional and structural recovery of ischemic skeletal muscles. Limb ischemia was created in Sprague-Dawley rats by excision of femoral and popliteal arteries. Allogeneic rat PKH26-labeled UCMSCs were administered by direct intramuscular injection. Animals of experimental group responded to the transplantation by improvement in their locomotor function as assessed by the rotarod performance test on day 9 and 29 after transplantation. Histomorphometric analysis showed that relative area of the lesions in the experimental group was significantly smaller than in the control group at all time points during the observation. Calculated densities of microcirculation vessels within the lesions were significantly higher in the experimental group than in the control group on day 10 after transplantation. Only a part of the transplanted allogeneic UC-MSCs survived within the ischemic muscle tissue, and a considerable portion of these surviving cells were found alongside the VEGFproducing preserved muscle fibers. The РКН26 label was not found within the walls of capillaries or larger blood vessels. The administration of allogeneic UCMSCs significantly increased the proportion of M2 macrophages, exhibiting proangiogenic and antiinflammatory properties, for at least 10 days following the transplantation
Autor/es principal/es: Arutyunyan, Irina
Fatkhudinov, Timur
Elchaninov, Andrey
Vasyukova, Olesya
Makarov, Andrey
Usman, Natalia
Kananykhina, Evgeniya
Lokhonina, Anastasiya
Goldshtein, Dmitry
Bolshakova, Galina
Sukhikh, Gennady
URI: http://hdl.handle.net/10201/121643
DOI: DOI: 10.14670/HH-18-057
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 11
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Vol.34, nº5 (2019)

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