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DOI: 10.14670/HH-18-051
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Título: | Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels |
Fecha de publicación: | 2019 |
Editorial: | Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and Histopathology, Vol.34, nº5, (2019) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Port wine stain Vascular malformations Endothelial cells Extracellular vesicle Exocytosis SWATH-MS |
Resumen: | Introduction. Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. Methods. Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM). Results. 107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/ exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control. Conclusions. The biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS. |
Autor/es principal/es: | Yin, Rong Rice, Shawn J. Wang, Jinwei Gao, Lin Tsai, Joseph Anvari, Radean T. Zhou, Fang Liu, Xin Wang, Gang Tang, Yuxin Mihm Jr, Martin C. Belani, Chandra P. Chen, Dong Bao Nelson, J. Stuart Tan, Wenbin |
URI: | http://hdl.handle.net/10201/121606 |
DOI: | DOI: 10.14670/HH-18-051 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 12 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.34, nº5 (2019) |
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Yin-34-479-490-2019.pdf | 12,49 MB | Adobe PDF | Visualizar/Abrir |
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