Por favor, use este identificador para citar o enlazar este ítem: DOI: 10.14670/HH-18-051

Título: Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels
Fecha de publicación: 2019
Editorial: Universidad de Murcia. Departamento de Biología Celular e Histología
Cita bibliográfica: Histology and Histopathology, Vol.34, nº5, (2019)
ISSN: 1699-5848
0213-3911
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología
Palabras clave: Port wine stain
Vascular malformations
Endothelial cells
Extracellular vesicle
Exocytosis
SWATH-MS
Resumen: Introduction. Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. Methods. Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM). Results. 107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/ exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control. Conclusions. The biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS.
Autor/es principal/es: Yin, Rong
Rice, Shawn J.
Wang, Jinwei
Gao, Lin
Tsai, Joseph
Anvari, Radean T.
Zhou, Fang
Liu, Xin
Wang, Gang
Tang, Yuxin
Mihm Jr, Martin C.
Belani, Chandra P.
Chen, Dong Bao
Nelson, J. Stuart
Tan, Wenbin
URI: http://hdl.handle.net/10201/121606
DOI: DOI: 10.14670/HH-18-051
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 12
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Vol.34, nº5 (2019)

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