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DOI: 10.14670/HH-11-961
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Simental Mendía, Mario | - |
dc.contributor.author | Vilchez Cavazos, Félix | - |
dc.contributor.author | García Garza, Rubén | - |
dc.contributor.author | Lara Arias, Jorge | - |
dc.contributor.author | Montes de Oca Luna, Roberto | - |
dc.contributor.author | Said Fernández, Salvador | - |
dc.contributor.author | Martínez Rodríguez, Herminia G. | - |
dc.date.accessioned | 2022-04-26T09:12:05Z | - |
dc.date.available | 2022-04-26T09:12:05Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Histology and Histopathology, Vol.33, nº6, (2018) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/119125 | - |
dc.description.abstract | Objective. To determine the effects of autologous leukocyte-poor platelet-rich plasma (LPPRP) on the expression of markers involved in cartilageextracellular matrix production and inflammation in cartilage explants bearing osteoarthritis. Materials and Methods. Cartilage explants and LP-PRP were obtained from 10 patients who underwent total knee arthroplasty. The explants were cultured in spinner flasks for 28 days in the presence of interleukin (IL)-1β and/or LP-PRP. The gene expression of catabolic (MMP13, ADAMTS5, and IL1β) and anabolic factors (COL2A1, ACAN, and SOX9) was quantified. A histological assessment was performed according to a modified Mankin score, and quantification of type II and I collagen deposition. Results. The gene expression of catabolic factors and the Mankin score were lower in LP-PRP- and LP-PRP/IL1β- than in IL-1β-treated explants, suggesting less matrix degradation in explants cultured in the presence of LP-PRP. Higher expression of genes involved in cartilage matrix restoration was observed in LP-PRP and LP-PRP/IL-1β- when compared to IL-1β-treated explants. The explants treated with LP-PRP and LPPRP/IL-1β exhibited a higher deposition of type II collagen as well as a lower deposition of type I collagen and also better surface integrity and a significant increase in the number of chondrocytes. Conclusion. LPPRP treatment favored restoration in early osteoarthritic cartilage and reduced the pro-inflammatory effect of IL1β. LP-PRP is a promising therapy for early osteoarthritis, as it promotes extracellular matrix repair, reduces inflammation, and slows cartilage degeneration. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Leukocyte-poor platelet-rich plasma | es |
dc.subject | Cartilage explants | es |
dc.subject | Extracellular matrix | es |
dc.subject | Interleukin-1β | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | The matrix synthesis and anti-inflammatory effect of autologous leukocyte-poor platelet rich plasma in human cartilage explants | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | DOI: 10.14670/HH-11-961 | - |
Aparece en las colecciones: | Vol.33, nº6 (2018) |
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Simental-Mendia-33-609-618-2018.pdf | 8,58 MB | Adobe PDF | Visualizar/Abrir |
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