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DOI: 10.14670/HH-11-838
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Otsuki, Shuhei | - |
dc.contributor.author | Murakami, Tomohiko | - |
dc.contributor.author | Okamoto, Yoshinori | - |
dc.contributor.author | Hoshiyama, Yoshiaki | - |
dc.contributor.author | Oda, Shuhei | - |
dc.contributor.author | Neo, Masashi | - |
dc.date.accessioned | 2022-02-24T11:59:19Z | - |
dc.date.available | 2022-02-24T11:59:19Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Histology and Histopathology, Vol.32, nº7, (2017) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/117368 | - |
dc.description.abstract | We previously reported that heparan sulfate 6-O endosulfatases (Sulfs) were expressed in articular cartilage, and that the Sulf-1 knockout mouse developed severe knee osteoarthritis. In this study, we hypothesised that intra-articular injection of Sulf-1 would prevent cartilage degeneration. After confirming that 1 mg/ml Sulf-1 did not induce ATDC5 cell death in vitro, gene expression of type II collagen and matrix metalloproteinase (MMP)-13 in the presence of Sulf-1 (1-100 ng/ml) were determined by quantitative real-time polymerase chain reaction. Sulf-1 was also injected intra-articularly into mice following surgical destabilisation of the medial meniscus to produce a model of osteoarthritis, and cartilage degeneration was evaluated by safranin O and MMP-13 staining. We also investigated fibroblast growth factor 2 (FGF2)/ extracellular signal-regulated kinase (Erk) cell signalling by western blotting. Exposure to Sulf-1 in vitro increased type II collagen expression and decreased MMP-13 expression in a concentration-dependent manner. Sulf-1 injection into the mouse osteoarthritic knee significantly suppressed glycosaminoglycan loss and MMP-13 expression. Erk1/2 signalling pathway activation was significantly reduced by Sulf-1 and FGF2. These findings indicate that Sulf-1 prevents cartilage degeneration by suppressing MMP-13 via an effect on FGF2/Erk1/2 signalling. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cartilage degeneration | es |
dc.subject | Sulf-1 | es |
dc.subject | FGF2 | es |
dc.subject | Erk1/2 | es |
dc.subject | MMP-13 | es |
dc.subject | Early osteoarthritis | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Suppression of cartilage degeneration by intra-articular injection of heparan sulfate 6-O endosulfatase in a mouse osteoarthritis model | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | DOI: 10.14670/HH-11-838 | - |
Aparece en las colecciones: | Vol.32, nº7 (2017) |
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Otsuki-32-725-733-2017.pdf | 13,29 MB | Adobe PDF | Visualizar/Abrir |
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