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DOI: 10.14670/HH-11-828
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Título: | Ghrelin and adipose-derived mesenchymal stromal cells improve nerve regeneration in a rat model of epsilon-caprolactone conduit reconstruction |
Fecha de publicación: | 2017 |
Editorial: | Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and Histopathology, Vol.32, nº6, (2017) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Epsilon caprolactone Ghrelin Peripheral nerve injury Nerve regeneration Rat Sciatic nerve |
Resumen: | Objective. Attempts have been made to improve nerve conduits in peripheral nerve reconstruction. We investigated the potential therapeutic effect of adipose-derived mesenchymal cells (ASCs) and ghrelin (GHR), a neuropeptide with neuroprotective, trophic, and developmental regulatory actions, on peripheral nerve regeneration in a model of severe nerve injury repaired with nerve conduits. Material and methods. The right sciatic nerves of 24 male Wistar rats were 10-mm transected unilaterally and repaired with Dl-lactic-ε-caprolactone conduits. Rats were then treated locally with saline, ASCs, or GHR. At 12 weeks post-surgery, we assessed limb function by measuring ankle stance angle and percentage muscle mass reduction and evaluated the histopathology, immunohistochemistry, ultrastructure, and morphometry of myelinated fibers. Main Results. Rats receiving GHR or ASCs showed no significant increased functional recovery in ankle stance angle (p=0.372) but a higher nerve area (p=0.015), myelin area (p=0.046) and number of myelinated fibers (p=0.012) in the middle and distal segments of operated sciatic nerves in comparison to saline-treated control animals. Conclusion. These results suggest that utilization of ghrelin or ASCs may improve nerve regeneration using Dl-lactic-ε-caprolactone conduits. |
Autor/es principal/es: | Hernández Cortés, Pedro Toledo Romero, Miguel Angel Delgado, Mario Gonzalez Rey, Elena Gómez Sánchez, Rafael Prados Olleta, Nicolás Aneiros Fernández, José Crespo Lora, Vicente Aguilar, Mariano Galindo Moreno, Pablo O’Valle, Francisco |
URI: | http://hdl.handle.net/10201/117324 |
DOI: | DOI: 10.14670/HH-11-828 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 11 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.32, nº6 (2017) |
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Fichero | Descripción | Tamaño | Formato | |
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Hernandez-Cortes-32-627-639-2017.pdf | 13,75 MB | Adobe PDF | Visualizar/Abrir |
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