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DOI: 10.14670/HH-11-770
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Min, He-ming | - |
dc.contributor.author | Wang, Yan | - |
dc.contributor.author | Ren, Da Yong | - |
dc.contributor.author | Cheng, Xue | - |
dc.contributor.author | Li, Jian | - |
dc.contributor.author | Jiang, Xing qian | - |
dc.contributor.author | Min, Lian qiu | - |
dc.contributor.author | Bao, Cui fen | - |
dc.date.accessioned | 2022-02-02T12:48:44Z | - |
dc.date.available | 2022-02-02T12:48:44Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Histology and Histopathology, Vol.32, nº1, (2017) | es |
dc.identifier.uri | http://hdl.handle.net/10201/116671 | - |
dc.description.abstract | We observed the effect of 2-deoxy-D-glucose (2-DG) on the brain tissue in rat cerebral ischemiareperfusion (I/R) and explored its mechanism. After observing the effect of 2-DG on endoplasmic reticulum stress (ERS), rats were randomly divided into shamoperation group, I/R group and I/R+2-DG group (each group with 60 rats). I/R models were prepared by middle cerebral artery occlusion. In I/R+2-DG group, each rat was given intraperitoneal 2-DG of 100 mg/kg once a day for 7 days before brain ischemia. According to different time points (3 h, 6 h, 12 h, 24 h and 48 h) after I/R, each group was divided into 5 subgroups (each subgroup with 12 rats). Nerve cell apoptosis, and the expressions of mRNA and protein of glucose regulated protein 78 (GRP78), cleaved-caspase-9 and cleaved-caspase-3 were determined with TUNEL, Western blotting and RT-PCR, respectively, in rat cerebral hippocampal CA1 area at each time point. TUNEL-positive cells were significantly less in I/R+2-DG group than in I/R group at each time point (all P<0.01). In I/R and I/R+2-DG groups, the expressions of mRNA and protein of GRP78 reached the maximum 12 h after I/R, and cleavedcaspase-9 and cleaved-caspase-3 reached the maximum 24 h after I/R. Compared with sham-operation group, the expressions of mRNA and protein of GRP78, cleavedcaspase-9 and cleaved-caspase-3 were all significantly increased (all P<0.01) in I/R and I/R+2-DG groups. However, the expressions of mRNA and protein of GRP78 were significantly higher in I/R+2-DG group than in I/R group (all P<0.05), but the expressions of mRNA and protein of cleaved-caspase-9 and cleavedcaspase-3 were all significantly lower in I/R+2-DG group than in I/R group (all P<0.05). We conclude that 2-DG has a neuroprotective effect on the brain tissue in rat cerebral ischemia-reperfusion models. The mechanism may be that 2-DG starts ERS followed by up-regulation of mRNA and protein of GRP78 and down-regulation of mRNA and protein of cleavedcaspase-9 and cleaved-caspase-3, which blocks the apoptotic pathway. | es |
dc.format | application/pdf | es |
dc.format.extent | 11 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | 2-deoxy-D-glucose | es |
dc.subject | Endoplasmic reticulum stress | es |
dc.subject | Cerebral ischemia-reperfusion injury | es |
dc.subject | Glucose regulated protein 78 | es |
dc.subject | Cleaved-caspase-9 | es |
dc.subject | Cleaved-caspase-3 | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Protective effect of 2-deoxy-D-glucose on the brain tissue in rat cerebral ischemia-reperfusion models by inhibiting caspase-apoptotic pathway | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | DOI: 10.14670/HH-11-770 | - |
Aparece en las colecciones: | Vol.32, nº1 (2017) |
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Min-32-57-67-2017.pdf | 20,99 MB | Adobe PDF | Visualizar/Abrir |
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