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Título: | Continuous intracellular pH measurement: Escherichia coli culture medium pH dependence |
Fecha de publicación: | 4-may-2021 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica |
Palabras clave: | Escherichia col pH intracellular fluorescence |
Resumen: | E. coli is the most used host microorganism to high-valuable compounds production in current industry. Cells pH homeostasis is a crucial factor to grow in different environment maintaining an intracellular pH control, however, to our knowledge, there is not a study about E. coli continuous intracellular pH growing in a culture. In this study the intracellular E. coli pH has been determined for cultures growing with glucose or glycerol as carbon sources. To determine intracellular E. coli pH, a novel, simple and accurate method based on pGFPR01 plasmid (kindly provided by Dr. Joan Slonczewski) was employed (1). This vector expressed ratiometric pHluorin. Thus, fluorescence ratio 410/470 showed a perfect lineal dependence with intracellular pH between 5.5 and 9. The results showed a surprising high intracellular pH for E. coli glucose cultures, which raised values of 8.5-9 at exponential growth phase. However, glycerol cultures showed a major regulation of intracellular pH, keeping the values at 6.5-7.5 in exponential and stationary stages. These differences show the dependence between the intracellular pH and the carbon source. In addition, the measurement method developed is a very useful tool for the control of bioprocesses. |
Autor/es principal/es: | Gallego Jara, Julia Lozano Terol, Gema Cánovas Díaz, Manuel de Diego Puente, Teresa |
Facultad/Departamentos/Servicios: | Department of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia |
Forma parte de: | 13th European Symposium on Biochemical Engineering Sciences 2021 |
URI: | http://hdl.handle.net/10201/113699 |
Tipo de documento: | info:eu-repo/semantics/other |
Número páginas / Extensión: | 1 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Ponencias y comunicaciones |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Poster ESBES 2021.pdf | 419,13 kB | Adobe PDF | Visualizar/Abrir |
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