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DOI: 10.14670/HH-11-739
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Unverdorben, Laura | - |
dc.contributor.author | Jeschke, Udo | - |
dc.contributor.author | Santoso, Laura | - |
dc.contributor.author | Hofmann, Simone | - |
dc.contributor.author | Kuhn, Christina | - |
dc.contributor.author | Arck, Petra | - |
dc.contributor.author | Hutter, Stefan | - |
dc.date.accessioned | 2021-10-29T08:23:11Z | - |
dc.date.available | 2021-10-29T08:23:11Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Histology and Histopathology, Vol.31, nº10, (2016) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/113523 | - |
dc.description.abstract | Introduction: Galectins are members of the mammalian β-galactoside-binding proteins, which recognize Galβ1-4GlcNAc sequences of several cell surface oligosaccharides. Plenty of galectins are already described in human tissue, especially in placenta. Here, gal-1-4, 7-10 and gal-12 were investigated systematically in trophoblast and decidua cells of first trimester placentas. Material and methods: Within this study, 15 first trimester placentas after induced abortion (7th-14th week of gestation) were examined with immunohistology and immunofluorescence based on a scoring system. Moreover, isolated and cultivated trophoblast cells from the first trimester were analyzed and evaluated for expression of gal-1-4, gal-7-10 and gal-12 at mRNA and protein level with real-time RTPolymerase chain Reaction/PCR (Taq-Man). Double immunofluorescence with trophoblast specific markers identified galectin expressing cells at the feto-maternal interface. Results: We could detect immunohistochemical staining of galectins 1-4, 7-10 and 12 in first trimester placenta: all examined galectins were found in the cytotrophoblast (CTB) and syncytiotrophoblast (SCT). Gal-1, -2, -3, -4, -7, -8, -9, -10 and -12 were identified in extravillous trophoblast cells (EVT) in immunohistology and immunoflourescence. The expression of gal-1, -9, - 10, and gal-12 increased after 96h incubation in vitro without stimulation at mRNA level, while gal-2, -3, -4, -7 and -8 were decreased. Discussion and conclusion: This study describes a systematic analysis of the expression of gal-1-4, gal-7-10 and gal-12 in first trimester placentas and isolated trophoblast cells. Expression levels at mRNA level and the change within 96h cultivation in vitro indicate a possible influence on syncytium building of trophoblast cell on expression of galectins. Therefore, an interaction of galectins in vitro in syncytium building is possible. | es |
dc.format | application/pdf | es |
dc.format.extent | 17 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Galectins | es |
dc.subject | Placenta | es |
dc.subject | Tophoblast | es |
dc.subject | Decidua | es |
dc.subject | First trimester | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Comparative analyses on expression of galectins1-4, 7-10 and 12 in first trimester placenta, decidua and isolated trophoblast cells in vitro | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | DOI: 10.14670/HH-11-739 | - |
Aparece en las colecciones: | Vol.31,nº 10 (2016) |
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Unverdorben-31-1095-1111-2016.pdf | 19,17 MB | Adobe PDF | Visualizar/Abrir |
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