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doi:10.1016/j.bej.2007.10.013
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Título: | PRODUCTION OF RICINOLEIC ACID ESTOLIDE WITH FREE AND IMMOBILIZED LIPASE FROM Candida rugosa. |
Fecha de publicación: | 23-oct-2007 |
Palabras clave: | Estolide ricinoleic acid lipase immobilized enzymes heterogeneous biocatalysis |
Resumen: | Ricinoleic acid estolide was produced by using free and immobilized Candida rugosa lipase at moderate temperature in a bioreactor. This work describes the immobilization of Candida rugosa lipase on ten different supports by covalent binding and physical adsorption, and how of the most suitable immobilized derivative was selected. The comparison was mainly based on the enzyme content and on the activity results. An anion exchange resin was judged to be the most appropriate support and the corresponding immobilization process was investigated and optimized. Although repeated batch reactions using the same derivative are not entirely advisable, the reaction proceeds at a noticeably slower rate and the degree of condensation reached is lower when the same amount of protein as in the derivative is added to the bioreactor in native form. |
Autor/es principal/es: | Bódalo, A. Bastida Rodríguez, Josefa Máximo Martín, María Fuensanta Montiel Morte, María Claudia Gómez, M. Murcia Almagro, María Dolores |
URI: | http://hdl.handle.net/10201/113070 |
DOI: | doi:10.1016/j.bej.2007.10.013 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 19 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Artículos: Ingeniería Química |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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BEJ 2008.pdf | 171,86 kB | Adobe PDF | Visualizar/Abrir |
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