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DOI: 10.14670/HH-11-719
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Título: | Analysis of cell-cell junctions in human amnion and chorionic plate affected by chorioamnionitis |
Fecha de publicación: | 2016 |
Cita bibliográfica: | Histology and histopathology, Vol.31, nº7 (2016) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Tight junction Adherent junction Amnion Chorioamnionitis |
Resumen: | Chorioamnionitis is an acute inflammatory reaction associated with the premature rupture of the fetal membranes. It is caused mainly by invasion of bacteria from the vaginal tract that can penetrate the intact membranes and invade the amnion cavity and the decidua. Tight junctions (TJ) and adherent junctions (AJ) are intercellular junctions crucial for epithelia adhesion and permeability regulation in a wide variety of tissues and organs. Our aim is to investigate if TJ and AJ molecules are involved in human chorioamnionitis. We studied the protein expression (by immunohistochemistry and western blotting) and the mRNA levels (by RT-PCR) of some junction proteins such as Zonula Occludens-1 (ZO-1), occludin, VE-cadherin and βcatenin in fetal membranes from women with chorioamnionitis compared to those membranes derived from idiopathic pregnancies. Western blotting and immunohistochemical data established that occludin expression was decreased in amnion with chorioamnionitis compared to amnion from idiopathic pregnancies. Samples tested for ZO-1, VEcadherin and β-catenin (proteins and mRNAs) showed no differences between idiopathic and pathological membranes. One of the most relevant results is the decrease of occludin in membranes with chorioamnionitis. Since we have previously demonstrated that some cytokines, particularly elevated in the chorioamnionitis, cause the disruption of TJs in placental villi, we suggest that the decrease of occludin in amnion may be the first change that leads to the rupture of the amniotic membrane in this pathology. |
Autor/es principal/es: | Licini, Caterina Tossetta, Giovanni Avellini, Chiara Ciarmela, Pasquapina Lorenzi, Teresa Toti, Paolo Gesuita, Rosaria Voltolini, Chiara Petraglia, Felice Castellucci, Mario Marzioni, Daniela |
URI: | http://hdl.handle.net/10201/111408 |
DOI: | DOI: 10.14670/HH-11-719 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.31, nº7 (2016) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Licini-31-759-767-2016.pdf | 3,62 MB | Adobe PDF | Visualizar/Abrir |
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