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DOI: 10.14670/HH-11-698
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Cayli, Sevil | - |
dc.contributor.author | Ocakli, Seda | - |
dc.contributor.author | Senel, Ufuk | - |
dc.contributor.author | Karaca, Zafer | - |
dc.contributor.author | Erdemir, Fikret | - |
dc.contributor.author | Delibasi, Tuncay | - |
dc.date.accessioned | 2021-07-05T08:57:59Z | - |
dc.date.available | 2021-07-05T08:57:59Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Histology and histopathology: Vol.31, nº5 (2016) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/110402 | - |
dc.description.abstract | Testicular torsion is a well-known medical emergency that can lead to pathological changes in the testicular tissues and male infertility. This investigation was undertaken to gain insight into the effects of an endothelin type A receptor antagonist (BQ123) on torsion-induced germ cell loss. Twenty-eight male Wistar albino rats were divided into four groups. In group I (control group), a sham operation to the left testis was performed. In group II (I/R injury), I/R injury was created by rotating the left testis 720° in a clockwise direction for 2 h and detorsing the testis after 2 h. In group III (I/R injury+BQ123), the rats were subjected to I/R injury and BQ123 injection (1 mg/kg, intravenous). In group IV (control+BQ123), the sham operated rats were subjected to BQ123. The testes of the rats were removed in all groups. Torsion-induced apoptosis and the effects of BQ123 were examined by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) technique, immunohistochemistry and western blotting. In group II, the number of TUNEL-positive cells increased after testicular torsion. Immunohistochemistry and western blotting showed that apoptotic proteins (active caspase 3 and Bax) were upregulated, and the anti-apoptotic protein Bcl2 was downregulated in I/R injury. The administration of BQ123 caused a significant decrease in the number of apoptotic cells and the expression of apoptotic proteins (p<0.05) when compared with the I/R injury group. No significant effect of BQ123 was observed in the testicular cells of group IV. This animal study provides evidence of the regulatory effects of BQ123 on torsion-induced testicular apoptosis. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Apoptosis | es |
dc.subject | Endothelin type A receptor antagonist | es |
dc.subject | Rat | es |
dc.subject | Testis | es |
dc.subject | Torsion | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Role of an endothelin type A receptor antagonist in regulating torsion-induced testicular apoptosis in rats | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | DOI: 10.14670/HH-11-698 | - |
Aparece en las colecciones: | Vol.31, nº5 (2016) |
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Fichero | Descripción | Tamaño | Formato | |
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Cayli-31-585-594-2016.pdf | 8,82 MB | Adobe PDF | Visualizar/Abrir |
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