Por favor, use este identificador para citar o enlazar este ítem: https://doi.org/10.15255/CABEQ.2014.19

Título: Biocatalytic Synthesis of Polyglycerol Polyricinoleate: A Comparison of Different Commercial Lipases
Fecha de publicación: oct-2013
Fecha de defensa / creación: 19-abr-2013
Editorial: Assoc. of Chemists and Chemical Engineers of Croatia
Cita bibliográfica: Chemical and Biochemical Engineering Quaterly, 2013, 27 (4) 439–448
Materias relacionadas: CDU::6 - Ciencias aplicadas
Palabras clave: Polyglycerol polyricinoleate
lipase
immobilized enzymes
heterogeneous reaction
enzyme biocatalysis
vacuum reactor
Resumen: In this work, the studies were carried out to select the most suitable lipase as catalyst for the esterification of polyglycerol with polyricinoleic acid to yield polyglicerol polyricinoleate (PGPR), a valued-added, biobased food emulsifier are described. The enzymes assayed were lipases from Rhizopus arrhizus, Rhizopus oryzae and Mucor javanicus, previously selected because of their suitable activity and moderate cost. First, reaction was catalyzed by free lipases in a batch reactor and the influence of different operating conditions (initial water content, amount of enzyme and temperature) on the progress of the reaction was studied. Next, the three lipases were immobilized by physical adsorption on the anion exchange resin, Lewatit MonoPlus MP 64, providing derivatives with a high activity and stability. Recovery of the immobilized derivative from the reaction medium was conducted with very good yields (up 99%) and no loss of activity of the derivative with successive uses was proved. Finally, a high performance reactor, which operates at low pressure and with a dry atmosphere, was used to synthesise PGPR using the immobilized enzymes. Both Rhizopus arrhizus and Rhizopus oryzae lipases allowed the production of a PGPR which fulfils the “specific purity criteria on food additives other than colours and sweeteners” established by the Commission of the European Communities, with an acid value of 4.91 and 5.31 mg KOH/g respectively.
Autor/es principal/es: Ortega, Salvadora
Gómez, José Luis
Bastida, Josefa
Máximo, Mª Fuensanta
Montiel, Mª Claudia
Gómez, María
Facultad/Departamentos/Servicios: Ingeniería Química
Publicado en: Nombre del proyecto: Biosíntesis de emulsionantes con aplicaciones alimentarias y cosméticas, en sistemas “solvent free”, utilizando lipasas comerciales. Código: CTQ2011-24091
Versión del editor: http://silverstripe.fkit.hr/cabeq/assets/Uploads/Cabeq-2013-04-07.pdf
URI: http://hdl.handle.net/10201/106685
DOI: https://doi.org/10.15255/CABEQ.2014.19
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 39
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Artículos: Ingeniería Química

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