Trophoblast induced spiral remodelling and uteroplacental haemodynamics in hypertensive pregnant rats with reduced heme oxygenase activity.

Abstract

Introduction The aim of the present study was to determine the contribution of the heme oxygenase (HO) system to the adaptation of the uteroplacental circulation to pregnancy in the rat, and its relationship with the maintenance of blood pressure during late gestation. Methods The HO inhibitor, stannous mesoporphyrin (SnMP), or vehicle were administered intraperitoneally to virgin and midpregnant rats. Mean arterial pressure (MAP) was measured before and after the treatment, in the conscious rats. Uterine and radial arteries blood flow velocities were obtained from pregnant rats at days 14 and 19 of gestation using high frequency ultrasonography. Trophoblast invasion and spiral arteries remodelling were analyzed in the mesometrial triangle of pregnant rats by inmunohistochemistry. Results HO activity inhibition during late gestation induced a significantly increase in the MAP of pregnant rats (114±1 mmHg vs 100±2 mmHg, p<0.05) but it did not affect this parameter in virgin rats (121±2 mmHg vs 124±3 mmHg). MAP elevation was associated with marked (p<0.05) decreases in the systolic and diastolic flow velocities in uterine and radial arteries, as compared with pregnant control rats. Furthermore, spiral arteries of pregnant rats treated with SnMP showed lower (p<0.001) proportion of lumen circumference covered by trophoblast (21±3%) and a higher (p<0.05) proportion of vascular smooth muscle (33±5%) than control pregnant rats (59±5% and 16±5%, respectively) Discussion These data indicate that HO system play an important role in the adaptation of the uteroplacental circulation to pregnancy and in the blood pressure regulation during late gestation.