Por favor, use este identificador para citar o enlazar este ítem: 10.1111/and.13148

Título: Lectin binding pattern of glycoconjugates during spontaneous testicular recrudescence in Syrian hamster (Mesocricetus auratus) after exposure to short photoperiod
Fecha de publicación: 1-feb-2019
Editorial: Wiley
Cita bibliográfica: Andrologia. 2019 Feb;51(1):e13148. doi: 10.1111/and.13148. Epub 2018 Sep 23.
Materias relacionadas: CDU::6 - Ciencias aplicadas
Palabras clave: . Glycoconjugates. Lectins. Hamster. Apoptosis. Recrudescence
Resumen: Lectin histochemistry was used to characterize glycoconjugates and cellular apoptosis in the seminiferous epithelium and interstitium of hamster testis during spontaneous recrudescence. An increase in the LTA lectin affinity was observed in spermatids in the Golgi phase. An increase in labelling of PNA and Con-A lectin in acrosome of spermatids (acrosome phase) as well increased labelling with Con-A in spermatids (cap phase) was observed. Spermatocytes showed decreased affinity with PNA and AAA lectins and an increase in positivity for LTA and GNA lectins. Spermatogonia showed a slight decrease in positivity to WGA and an increase in labelling with Con-A, and a decreased affinity for the AAA lectin. At the end of recrudescence all these germinal cells showed a similar pattern to the control. The Sertoli cells showed a gradual decrease in labelling with the GNA lectin and the Leydig cells an increase in labelling with Con-A and GNA. Particularly unusual was the observation of apoptotic spermatocytes and spermatids positive for PNA, GNA, AAA and Con-A, together with spermatocytes positive to LTA. In conclusion the normal lectin pattern is recovered during testis recrudescence and germ cell apoptotic activity is low, as is observed by specific lectins for germ cells in apoptosis.
Autor/es principal/es: Martínez-Hernández, Jesús
Seco-Rovira, Vicente
Beltran-Frutos, Ester
Ferrer, Concepción
Serrano-Sánchez, María Isabel
Pastor, Luis Miguel
Facultad/Departamentos/Servicios: Department of Cell Biology and Histology, Medical School, IMIB-Arrixaca, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Spain
Versión del editor: https://onlinelibrary.wiley.com/doi/full/10.1111/and.13148
URI: http://hdl.handle.net/10201/106562
DOI: 10.1111/and.13148
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 25
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Descripción: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Andrologia]. To access the final edited and published work see [10.1111/and.13148]
Aparece en las colecciones:Artículos: Biología Celular e Histología

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