Por favor, use este identificador para citar o enlazar este ítem: 10.1111/rda.13201

Título: Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster during spontaneous recrudescence after exposure to short photoperiod
Fecha de publicación: 1-oct-2018
Editorial: © 2018 Blackwell Verlag GmbH.
Cita bibliográfica: Reproduction in domestic animals . 2018 Oct;53(5):1041-1051
ISSN: 1439-0531
Materias relacionadas: CDU::6 - Ciencias aplicadas
Palabras clave: Testes, Photoperiod, Apoptosis, Proliferation, Recrudescence, Andrology.
Resumen: Syrian hamsters are photoperiodic rodents in which reproduction, including testicular function, is stimulated by long photoperiod exposure and curtailed by exposure to a short photoperiod. The objectives of this study were to characterize the testis histomorphometrically and to determine the role of the proliferation and apoptosis phenomena in the recovery of the seminiferous epithelium during spontaneous recrudescence after exposure to short photoperiod. The study was performed using conventional light microscopy, proliferating cell nuclear antigen and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP in situ nick end labelling staining, image analysis software, and transmission electron microscopy in three recrudescence groups: initial recrudescence (IR), advanced recrudescence (AR), and total recrudescence (TR). Morphometrically, the results pointed to the gradual recovery of the testicular and tubular volumes, as well as of the seminiferous epithelium. Among the IR and AR groups, the increase in testicular and tubular volumes was accompanied by an increase in tubular diameter and length, with an increase in interstitial volume. From AR to TR, there was an increase in the tubular and total volumes, but, in this case, with a gradual increase in tubular diameter. Recovery of the seminiferous epithelium was accompanied by changes in apoptosis and proliferation activities. The first decreased half way through the process and the second remained higher than the control levels throughout the recrudescence stage. Ultrastructurally, alterations in the spermatozoa were observed, which indicated that spermiogenesis was not yet completely normal. In conclusion, spontaneous testicular recrudescence in Syrian hamster comprises two histomorphometrical phases, the first related to an increase in tubular length and diameter and interstitial volume, and the second depending principally on the gradual increase in tubular diameter. The restoration of the seminiferous epithelium is due to apoptosis reaching normal values in the AR group accompanied by higher proliferative activity than that observed in the Control group.
Autor/es principal/es: Martínez-Hernández, Jesús
Seco-Rovira, Vicente
Beltran-Frutos, Ester
Ferrer, Concepción
Canteras, Manuel
Sánchez-Huertas, María del Mar
Pastor, Luis Miguel
Facultad/Departamentos/Servicios: 1Department of Cell Biology and Histology, Medical School, IMIB-Arrixaca, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Spain. 2Department of Statistics, Medical School, University of Murcia, Murcia, Spain.
Versión del editor: https://onlinelibrary.wiley.com/doi/full/10.1111/rda.13201
URI: http://hdl.handle.net/10201/106507
DOI: 10.1111/rda.13201
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 32
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Descripción: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Reproduction in domestic animals]. To access the final edited and published work see [10.1111/rda.13201].”
Aparece en las colecciones:Artículos: Biología Celular e Histología

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