Por favor, use este identificador para citar o enlazar este ítem: 10.1111/j.1600-0854.2004.00242.x

Título: Structure and Dynamics of the Golgi Complex at 15ºC: Low Temperature Induces the Formation of Golgi-Derived Tubules
Fecha de publicación: ene-2005
Fecha de defensa / creación: jun-2004
Editorial: Wiley
Palabras clave: endoplasmic reticulum-Golgi intermediate compartment
Golgi complex
immunoelectron microscopy
immunofluorescence
membrane traffic
Resumen: Immunofluorescence and cryoimmunoelectron microscopy were used to examine the morphological and functional effects on the Golgi complex when protein transport is blocked at the ERGIC (ER-Golgi intermediate compartment) in HeLa cells incubated at low temperature (15ºC). At this temperature, the Golgi complex showed long tubules containing resident glycosylation enzymes but not matrix proteins. These Golgi-derived tubules also lacked anterograde (VSV-G) or retrograde (Shiga toxin) cargo. The formation of tubules was dependent on both energy and intact microtubule and actin cytoskeletons. Conversely, brefeldin A or cycloheximide treatments did not modify the appearance. When examined at the electron microscope, Golgi stacks were long and curved and appeared connected to tubules immunoreactive to galactosyltransferase antibodies but devoid of Golgi matrix proteins. Strikingly, COPI proteins moved from membranes to the cytosol at 15ºC which could explain the formation of tubules.
Autor/es principal/es: Martínez-Alonso, Emma
Egea, Gustavo
Ballesta Germán, José
Martínez-Menárguez, José Ángel
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Biología Celular e Histología
URI: http://hdl.handle.net/10201/105644
DOI: 10.1111/j.1600-0854.2004.00242.x
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 37
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Artículos: Biología Celular e Histología

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