Por favor, use este identificador para citar o enlazar este ítem: 10.1016/j.brainresbull.2019.05.006

Título: Potential role of P2X7 receptor in neurodegenerative processes in a murine model of glaucoma
Fecha de publicación: 2019
Fecha de defensa / creación: 2019
Editorial: Elsevier
Cita bibliográfica: Brain Research Bulletin 150 (2019) 61-74
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina::617 - Cirugía. Ortopedia. Oftalmología
CDU::6 - Ciencias aplicadas::61 - Medicina::612 - Fisiología
Palabras clave: Glaucoma
Retinal ganglion cells (RGC)
Neuronal degeneration
Apoptosis
Electroretinogram (ERG)
P2X7
MAPK
Resumen: Glaucoma is a common cause of visual impairment and blindness, characterized by retinal ganglion cell (RGC) death. The mechanisms that trigger the development of glaucoma remain unknown and have gained significant relevance in the study of this neurodegenerative disease. P2X7 purinergic receptors (P2X7R) could be involved in the regulation of the synaptic transmission and neuronal death in the retina through different pathways. The aim of this study was to characterize the molecular signals underlying glaucomatous retinal injury. The time-course of functional, morphological, and molecular changes in the glaucomatous retina of the DBA/2J mice were investigated. The expression and localization of P2X7R was analysed in relation with retinal markers. Caspase-3, JNK, and p38 were evaluated in control and glaucomatous mice by immunohistochemical and western-blot analysis. Furthermore, electroretinogram recordings (ERG) were performed to assess inner retina dysfunction. Glaucomatous mice exhibited changes in P2X7R expression as long as the pathology progressed. There was P2X7R overexpression in RGCs, the primary injured neurons, which correlated with the loss of function through ERG measurements. All analyzed MAPK and caspase-3 proteins were upregulated in the DBA/2J retinas suggesting a pro-apoptotic cell death. The increase in P2X7Rs presence may contribute, together with other factors, to the changes in retinal functionality and the concomitant death of RGCs. These findings provide evidence of possible intracellular pathways responsible for apoptosis regulation during glaucomatous degeneration.
Autor/es principal/es: Pérez de Lara, María J.
Avilés-Trigueros, Marcelino
Guzmán-Aránguez, Ana
Valiente-Soriano, F. Javier
de la Villa, Pedro
Vidal-Sanz, Manuel
Pintor, Jesús
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
Versión del editor: https://www.sciencedirect.com/science/article/pii/S0361923018306622?via%3Dihub
URI: http://hdl.handle.net/10201/105630
DOI: 10.1016/j.brainresbull.2019.05.006
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 14
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Artículos: Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica

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