Por favor, use este identificador para citar o enlazar este ítem: 10.1074/jbc.M117.786087

Título: Differential functional regulation of protein kinase C (PKC) orthologs in fission yeast.
Fecha de publicación: jul-2017
Fecha de defensa / creación: 2017
Editorial: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Cita bibliográfica: The Journal of biological chemistry. 2017; 292 (27): 11374-11387
ISSN: 0021-9258
Materias relacionadas: CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología
Palabras clave: MAPK
PKC
Rho (Rho GTPase)
Schizosaccharomyces pombe
Signal Transduction
Stress Response
Resumen: The two PKC orthologs Pck1 and Pck2 in the fission yeast Schizosaccharomyces pombe operate in a redundant fashion to control essential functions, including morphogenesis and cell wall biosynthesis, as well as the activity of the cell integrity pathway (CIP) and its core element the MAPK Pmk1. We show here that despite the strong structural similarity and functional redundancy of these two enzymes, the mechanisms regulating their maturation, activation, and stabilization have a remarkably distinct biological impact on both kinases. We found that in contrast to Pck2, putative in vivo phosphorylation of Pck1 within the conserved activation loop, turn and hydrophobic motifs is essential for Pck1 stability and biological functions. Constitutive Pck activation promoted dephosphorylation and destabilization of Pck2, while it enhanced Pck1 levels to interfere with proper downstream signaling to the CIP via Pck2. Importantly, whereas catalytic activity was essential for Pck1 function, Pck2 remained partially functional independently of its catalytic activity. Our findings suggest that early divergence from a common ancestor in fission yeast involved important changes in the mechanisms regulating catalytic activation and stability of PKC family members to allow for flexible and dynamic control of downstream functions, including MAPK signaling.
Autor/es principal/es: Madrid, M.
Vázquez-Marín, B.
Soto, T.
Franco, A.
Gómez-Gil, E.
Vicente-Soler, J.
Gacto, M.
Pérez, P.
Cansado Vizoso, José
Facultad/Departamentos/Servicios: Genética y Microbiología
Versión del editor: https://www.sciencedirect.com/science/article/pii/S0021925820370083?via%3Dihub
URI: http://hdl.handle.net/10201/105025
DOI: 10.1074/jbc.M117.786087
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 13
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Artículos: Genética y Microbiología

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