Por favor, use este identificador para citar o enlazar este ítem: 10.14670/HH-11-642

Título: Chondrocyte differentiation for auricular cartilage reconstruction using a chitosan based hydrogel
Fecha de publicación: 2015
Editorial: Universidad de Murcia. Departamento de Biología Celular e Histología
Cita bibliográfica: Histology and Histopathology, vol. 30, nº12, (2015)
ISSN: 1699-5848
0213-3911
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología
Palabras clave: Auricular cartilage
Microtia
Chondrocyte differentiation
Chitosan hydrogel
Endochondral ossification
Resumen: Tissue engineering with the use of biodegradable and biocompatible scaffolds is an interesting option for ear repair. Chitosan-Polyvinyl alcohol-Epichlorohydrine hydrogel (CS-PVA-ECH) is biocompatible and displays appropriate mechanical properties to be used as a scaffold. The present work, studies the potential of CS-PVA-ECH scaffolds seeded with chondrocytes to develop elastic cartilage engineered-neotissues. Chondrocytes isolated from rabbit and swine elastic cartilage were independently cultured onto CS-PVA-ECH scaffolds for 20 days to form the appropriate constructs. Then, in vitro cell viability and morphology were evaluated by calcein AM and EthD-1 assays and Scanning Electron Microscopy (SEM) respectively, and the constructs were implanted in nu/nu mice for four months, in order to evaluate the neotissue formation. Histological analysis of the formed neotissues was performed by Safranin O, Toluidine blue (GAG’s), Verhoeff-Van Gieson (elastic fibers), Masson’s trichrome (collagen) and Von Kossa (Calcium salts) stains and SEM. Results indicate appropriate cell viability, seeded with rabbit or swine chondrocyte constructs; nevertheless, upon implantation the constructs developed neotissues with different characteristics depending on the animal species from which the seeded chondrocytes came from. Neotissues developed from swine chondrocytes were similar to auricular cartilage, while neotissues from rabbit chondrocytes were similar to hyaline cartilage and eventually they differentiate to bone. This result suggests that neotissue characteristics may be influenced by the animal species source of the chondrocytes isolated.
Autor/es principal/es: García-López, J.
Garciadiego-Cázares, D.
Melgarejo-Ramírez, Y.
Sánchez-Sánchez, R.
Solís-Arrieta, L.
García-Carvaja, Z.
Sánchez-Betancourt, J.I.
Ibarra, C.
Luna-Bárcena, G.
Velasquillo, C.
URI: http://hdl.handle.net/10201/103641
DOI: 10.14670/HH-11-642
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 9
Derechos: info:eu-repo/semantics/openAccess
Atribución-NoComercial-SinDerivadas 3.0 España
Aparece en las colecciones:Vol.30,nº12 (2015)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
Garcia-Lopez-30-1477-1485-2015.pdf19,22 MBAdobe PDFVista previa
Visualizar/Abrir


Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons