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10.14670/HH-11-630
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Grzesiak, Malgorzata | - |
dc.contributor.author | Mitan, Agata | - |
dc.contributor.author | Janik, Marcelina E. | - |
dc.contributor.author | Knapczyk-Stwora, Katarzyna | - |
dc.contributor.author | Slomczynska, Maria | - |
dc.date.accessioned | 2021-02-15T08:17:30Z | - |
dc.date.available | 2021-02-15T08:17:30Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Histology and Histopathology, vol. 30, nº11, (2015) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/103301 | - |
dc.description.abstract | This study examined whether flutamideinduced androgen deficiency during mid- and late pregnancy in pigs affected luteal expression of adherens junction protein, β-catenin, and its interactions with Ecadherin. Flutamide (50 mg/kg body weight) was administered into pregnant gilts between days 43-49 (GD50F), 83-89 (GD90F) or 101-107 (GD108F) of gestation. Corpora lutea (CLs) were obtained on day 50, 90 or 108 of pregnancy (n=8-11 per each group). Total β-catenin and E-cadherin expression was examined at mRNA (real-time PCR) and protein (Western blot) level. Moreover, subcellular β-catenin fractions were extracted and immunoblotted. Immunohistochemistry was used for β-catenin localization. To determine whether flutamide disturbs β-catenin/E-cadherin mutual interactions, coimmunoprecipitation using anti-β-catenin antibody was performed. Furthermore, phosphorylation of Ecadherin was assessed. Flutamide exposure led to decreased β-catenin mRNA expression in all examined groups (p<0.001 or p<0.01), but protein level was lower only in the GD90F and GD108F groups (p<0.05). Ecadherin mRNA (p<0.05 or p<0.01) and protein (p<0.05) levels were up-regulated in all flutamidetreated groups when compared to controls. β-catenin was predominantly found in membranes of luteal cells with no significant changes after antiandrogen treatment. βcatenin/E-cadherin complexes were more abundant in the GD90F (p<0.05) and GD108F (p<0.01) groups than in controls due to enhanced E-cadherin phosphorylation at serine 838/840 in those animals (p<0.05). Overall, although androgen deficiency affected β-catenin expression in the CL of pregnancy in pigs, a compensatory mechanism by enhanced interactions with E-cadherin is possible. Thus, androgen signaling via androgen receptors appears to be crucial in the regulation of luteal cells cross-talk. | es |
dc.format | application/pdf | es |
dc.format.extent | 12 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | β-catenin | es |
dc.subject | E-cadherin | es |
dc.subject | Flutamide | es |
dc.subject | Corpus luteum | es |
dc.subject | Pig | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Flutamide alters β-catenin expression and distribution, and its interactions with E-cadherin in the porcine corpus luteum of mid- and late pregnancy | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | 10.14670/HH-11-630 | - |
Aparece en las colecciones: | Vol.30,nº11 (2015) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Grzesiak-30-1341-1352-2015.pdf | 4,47 MB | Adobe PDF | Visualizar/Abrir |
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