Por favor, use este identificador para citar o enlazar este ítem: https://doi.org/10.1016/j.dyepig.2016.06.037

Título: Fluorescent Bioinspired Protein Labeling with Betalamic Acid. Derivatization and Characterization of Novel Protein-Betaxanthins
Fecha de publicación: oct-2016
Cita bibliográfica: Dyes and Pigments, 2016, 133, 458-466
ISSN: 0143-7208
Materias relacionadas: CDU::5 - Ciencias puras y naturales
Palabras clave: Betalains
Betalamic acid
Resumen: Betaxanthins are the water-soluble pigments that bestow yellow coloration to fruits, flowers and roots of plants of the Caryophyllales order and present autofluorescence after excitation with blue light. In this work, the semi-synthesis of betaxanthins derived from macromolecules is achieved for the first time by exploiting the reactivity of amine groups belonging to proteins. The synthesis of protein-betaxanthins is demonstrated by spectrophotometry and HPLC-ESI-TOF-MS mass analysis. The derivatization with betalamic acid was in a ratio 1:1 and yielded protein-betaxanthins yellow in color that exhibited fluorescent properties with a maximum excitation wavelength of 476 nm and a maximum emission wavelength of 551 nm. Moreover, staining can be started from purified betalamic acid or directly from raw red beet root extracts. The novel bioinspired labeling reaction allowed protein detection in conventional fluorescence scanning and imaging systems and opens a new perspective for betalamic acid derived molecules as fluorescent probes with multiple biological applications.
Autor/es principal/es: Juana, Cabanes
Fernando, Gandía-Herrero
Josefa, Escribano
Francisco, García-Carmona
Mercedes, Jiménez-Atiénzar
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular A
URI: http://hdl.handle.net/10201/103101
DOI: https://doi.org/10.1016/j.dyepig.2016.06.037
Tipo de documento: info:eu-repo/semantics/annotation
Número páginas / Extensión: 36
Derechos: info:eu-repo/semantics/openAccess
Atribución-NoComercial-SinDerivadas 3.0 España
Aparece en las colecciones:Artículos: Bioquímica y Biología Molecular "A"

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