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10.14670/HH-11-628
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Título: | Ex vivo and in vivo modulatory effects of umbilical cord Wharton’s jelly stem cells on human oral mucosa stroma substitutes |
Fecha de publicación: | 2015 |
Editorial: | Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and Histopathology, vol.30, nº 11, (2015) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Human Wharton’s jelly stem cells Bioengineered oral mucosa Extracellular matrix Epithelial-mesenchymal transition Tissue engineering oral mucosa |
Resumen: | Novel oral mucosa substitutes have been developed in the laboratory using human umbilical cord Wharton’s jelly stem cells -HWJSC- as an alternative cell source. In the present work, we have generated human oral mucosa substitutes with oral mucosa keratinocytes and HWJSC to determine the influence of these cell sources on stromal differentiation. First, acellular and cellular stroma substitutes and bilayered oral mucosa substitutes with an epithelial layer consisting of oral mucosa keratinocytes -OM samplesor HWJSC -hOM- were generated. Then, tissues were analyzed by light and electron microscopy, histochemistry and immunohistochemistry to quantify all major extracellular matrix components after 1, 2 and 3 weeks of ex vivo development, and OM and hOM were also analyzed after in vivo grafting. The results showed that bioengineered oral mucosa stromas displayed an adequate fibrillar mesh. Synthesis of abundant collagen fibers was detected in OM and hOM after 3 weeks, and in vivo grafting resulted in an increased collagen synthesis. No elastic or reticular fibers were found. Glycoprotein synthesis was found at the epithelialstromal layer when samples were grafted in vivo. Finally, proteoglycans, decorin, versican and aggrecan were strongly dependent on the in vivo environment and the presence of a well-structured epithelium on top. The use of HWJSC was associated to an increased synthesis of versican. These results confirm the usefulness of fibrinagarose biomaterials for the generation of an efficient human oral mucosa stroma substitute and the importance of the in vivo environment and the epithelialmesenchymal interaction for the adequate differentiation of the bioengineered stroma. |
Autor/es principal/es: | Alfonso-Rodríguez, C.A. González-Andrades, E. Jaimes-Parra, B.D. Fernández-Valadé, R. s Campos, A. Sánchez-Quevedo, M. C. Alaminos, M. Garzon, I. |
URI: | http://hdl.handle.net/10201/103006 |
DOI: | 10.14670/HH-11-628 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 12 |
Derechos: | info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España |
Aparece en las colecciones: | Vol.30,nº11 (2015) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Alfonso-Rodriguez-30-1321-1332-2015.pdf | 14,72 MB | Adobe PDF | ![]() Visualizar/Abrir |
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