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10.14670/HH-15.173
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Título: | The visualization of oxidant stress in tissues and isolated cells |
Fecha de publicación: | 2000 |
Editorial: | Francisco Hernández, Professor of Cell Biology, University of Murcia, Murcia, Spain |
Cita bibliográfica: | Histology and Histopathology, vol. 15, nº 1, (2000) |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Oxidant stress Histochemical detection Marker ROS |
Resumen: | Many studies have implicated the role of oxidant stress in a wide range of human diseases and have led to the rapid expansion of research in this area. With many experimental approaches a direct detection of the production of reactive oxygen species (ROS) and free radicals is not possible. Free radicals are very reactive, short-lived and react in a non-specific way, so that ongoing oxidative damage is generally analyzed by measurement of secondary products e.g. H2O2, "oxidized" proteins, peroxidized lipids and their breakdown products, "oxidized" DNA or by fluorographic analysis in combination with fluorescent dyes e.g. dichlorofluorescin (DCFH). The histochemical visualization of selected molecular markers for oxidative phenomena can often provide valuable information concerning the distribution of oxidative processes in vivo. A number of biochemical methods are available for the monitoring of almost all oxidant stress-related processes, although their applicability in vivo is limited. This review summarizes the biochemical methods currently available for histochemical detection and indirect visualization of an excess of free radicals and ROS. The cited methods are discussed and the results obtained from their application are critically evaluated. |
Autor/es principal/es: | Frank, J. Biesalsk, H. K. Dominici, S. Pompella, A. |
URI: | http://hdl.handle.net/10201/92231 |
DOI: | 10.14670/HH-15.173 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 12 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International |
Aparece en las colecciones: | Vol.15, nº 1 (2000) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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The visualization of oxidant stress in tissues and isolated cells.pdf | 1,83 MB | Adobe PDF | Visualizar/Abrir |
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