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https://doi.org/10.1016/j.jaci.2018.05.040
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Título: | Artemisia pollen is the main vector for airborne endotoxin |
Fecha de publicación: | 9-ago-2018 |
Editorial: | Elsevier |
ISSN: | Print: 0091-6749 Electronic: 1097-6825 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología CDU::5 - Ciencias puras y naturales::57 - Biología CDU::5 - Ciencias puras y naturales::58 - Botánica |
Palabras clave: | Microbiology Bioaerosol Endotoxin Immunology Pseudomonas Ambient Bacteria Pollen Gram-negative Recombinant Factor c Davos Munich Artemisia species Microbiome Mouse model Allergy LPS Source PM10 PM2.5 |
Resumen: | Background: Endotoxin (LPS) released from gram-negative bacteria causes strong immunologic and inflammatory effects and, when airborne, can contribute to respiratory conditions, such as allergic asthma. Objectives: We sought to identify the source of airborne endotoxin and the effect of this endotoxin on allergic sensitization. Methods: We determined LPS levels in outdoor air on a daily basis for 4 consecutive years in Munich (Germany) and Davos (Switzerland). Air was sampled as particulate matter (PM) greater than 10 μm (PM > 10) and PM between 2.5 and 10 μm. LPS levels were determined by using the recombinant Factor C assay. Results: More than 60% of the annual endotoxin exposure was detected in the PM > 10 fraction, showing that bacteria do not aerosolize as independent units or aggregates but adhered to large particles. In Munich 70% of annual exposure was detected between June 12th and August 28th. Multivariate modeling showed that endotoxin levels could be explained by phenological parameters (ie, plant growth). Indeed, days with high airborne endotoxin levels correlated well with the amount of Artemisia pollen in the air. Pollen collected from plants across Europe (100 locations) showed that the highest levels of endotoxin were detected on Artemisia vulgaris (mugwort) pollen, with little on other pollen. Microbiome analysis showed that LPS concentrations on mugwort pollen were related to the presence of Pseudomonas species and Pantoea species communities. In a mouse model of allergic disease, the presence of LPS on mugwort pollen was needed for allergic sensitization. Conclusions: The majority of airborne endotoxin stems from bacteria dispersed with pollen of only one plant: mugwort. This LPS was essential for inducing inflammation of the lung and allergic sensitization. |
Autor/es principal/es: | Oteros, José Bartusel, Elke Alessandrini, Francesca Núñez, Andrés Moreno, Diego A. Behrendt, Heidrun Schmidt-Weber, Carsten Traidl-Hoffmann, Claudia Buters, Jeroen |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Genética y Microbiología |
URI: | http://hdl.handle.net/10201/143283 |
DOI: | https://doi.org/10.1016/j.jaci.2018.05.040 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 14 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2018 American Academy of Allergy, Asthma & Immunology. This document is the Published version of a Published Work that appeared in final form in Journal of Allergy and Clinical Immunology. To access the final edited and published work see https://doi.org/10.1016/j.jaci.2018.05.040 |
Aparece en las colecciones: | Artículos: Genética y Microbiología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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1-s2.0-S0091674918309990-main (1).pdf | Oteros2019 | 2,17 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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