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https://doi.org/10.1016/j.abb.2011.05.019
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Título: | Passive Ca(2+) overload in H9c2 cardiac myoblasts: assessment of cellular damage and cytosolic Ca(2+) transients |
Fecha de publicación: | 15-ago-2011 |
Editorial: | Elsevier |
Cita bibliográfica: | Archives of Biochemistry and Biophysics, 2011, Vol. 512, Issue 2, pp. 175-182 |
ISSN: | Print: 0003-9861 Electronic: 1096-0384 |
Palabras clave: | Sarcoplasmic reticulum Thapsigargin Ca2+ overload Ca2+ transient Cell damage H9c2 cell |
Resumen: | Increase of resting Ca(2+) levels and amplitude of vasopressin-induced Ca(2+) transients were observed when cells in serum-free medium were exposed to 5mM Ca(2+) for 2h. Small effect on cell viability was also observed. A rapid cytotoxic effect was developed in the presence of 10mM Ca(2+) and absence of serum. However, cells exposed to 10mM Ca(2+) in the presence of serum were protected from damage for at least 2days. Resting Ca(2+) levels and cytosolic Ca(2+) transients in serum-containing medium with 10mM Ca(2+) displayed lower increases and a tendency to recover control values. When serum was absent, cells preincubated with 10mM Ca(2+) were more sensitive to thapsigargin-induced damage than cells preincubated with lower Ca(2+). The sensitivity was similar when serum was present. Tolerance to high Ca(2+) in the presence of serum was linked to potentiation of the mitochondrial Ca(2+) entry to decrease the sarcoplasmic reticulum Ca(2+) overload. |
Autor/es principal/es: | Soler, Fernando Lax Pérez, Antonio Manuel Asensio López, María del Carmen Pascual Figal, Domingo Andrés Fernandez Belda, Francisco |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina Interna |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0003986111001913#aep-acknowledgment-id19 |
URI: | http://hdl.handle.net/10201/143112 |
DOI: | https://doi.org/10.1016/j.abb.2011.05.019 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2011 Elsevier Inc. This document is the Published version of a Published Work that appeared in final form in Archives of Biochemistry and Biophysics. To access the final edited and published work see https://doi.org/10.1016/j.abb.2011.05.019 |
Aparece en las colecciones: | Artículos: Medicina |
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