Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.14670/HH-18-472
Twittear
Título: | CircRNF220 plays a pathogenic role to facilitate cell progression of AML in vitro via sponging miR-330-5p to induce upregulation of SOX4 |
Fecha de publicación: | 2022 |
Editorial: | Universidad de Murcia, Departamento de Biologia Celular e Histiologia |
Cita bibliográfica: | Histology and Histopathology Vol. 37, nº10 (2022) |
ISSN: | 0213-3911 1699-5848 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | circRNF220 Acute myeloid leukemia miR-330-5p SOX4 |
Resumen: | Background: Circular RNAs (circRNAs) are a specific family of non-coding RNAs (ncRNAs) with important function in disease progression. This research is performed to study circRNA Ring Finger Protein 220 (circRNF220) in acute myeloid leukemia (AML). Methods: CircRNF220, microRNA-330-5p (miR330-5p) and sex-determining region Y-related highmobility group box 4 (SOX4) were measured via quantitative real-time polymerase chain reaction (qRTPCR). 3-(4, 5-dimethylthiazol-2-y1)-2, 5- diphenyl tetrazolium bromide (MTT) and EdU assays were used to assess cell proliferation. Cell cycle and apoptosis were detected using flow cytometry. Cell invasion was determined by transwell assay. Glycolytic metabolism was assessed by glucose consumption and lactate production. The target interaction was implemented via dual-luciferase reporter and RNA pull-down assays. SOX4 protein detection was conducted by western blot. Results: Expression detection identified that circRNF220 was overexpressed in AML. In vitro experiments showed that silence of circRNF220 promoted cell apoptosis but impeded proliferation, cell cycle progression, invasion and glycolytic metabolism in AML cells. Target analysis indicated that circRNF220 directly targeted miR-330-5p, and the effects of sicircRNF220 were abrogated by miR-330-5p inhibitor. Moreover, circRNF220 targeted miR-330-5p to increase the expression of SOX4 and SOX4 promoted cell progression of AML. Conclusion: All these findings revealed that circRNF220 contributed to AML cell development in vitro via upregulating SOX4 expression by targeting miR-330-5p. |
Autor/es principal/es: | Zhang, Zewen Lin, Shujun Yin, Jun Yu, Wenjun Xu, Chengwei |
URI: | http://hdl.handle.net/10201/128985 |
DOI: | https://doi.org/10.14670/HH-18-472 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 12 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.37,nº10 (2022) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Zhang-37-1019-1030-2022.pdf | 8,81 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons