Publication: Expression of EMT inducers integrin-linked kinase (ILK) and ZEB1 in phyllodes breast tumors is associated with aggressive phenotype
Authors
Akrida, Ioanna ; Nikou, Sofia ; Gyftopoulos, Konstantinos ; Argentou, Marianna ; Kounelis, Sofia ; Zolota, Vassiliki ; Bravou, Vasiliki ; Papadaki, Helen
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-987
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info:eu-repo/semantics/article
Description
Abstract
Phyllodes tumors (PTs) of the breast
constitute an uncommon group of mammary
fibroepithelial lesions with ambiguous biologic behavior.
Recent evidence suggests that epithelial mesenchymal
transition (EMT), a driving force of cancer progression
is implicated in PTs pathogenesis. Integrin-linked kinase
(ILK), a focal adhesion kinase, has been implicated in
cancer and EMT and represents a novel cancer
therapeutic target. In this study, we aimed to investigate
ILK and EMT markers expression in phyllodes breast
tumors in relation to tumor grade. Expression of ILK and
EMT markers E-cadherin, β-catenin, Ν-cadherin,
vimentin, Snail, ZEB1 and Twist was evaluated by
immunohistochemistry in paraffin-embedded tissue
sections from 96 human phyllodes breast tumors (48
benign, 27 borderline, 21 malignant). Cytoplasmic and
nuclear immunopositivity of ILK were observed in both
the epithelial and the stromal component of phyllodes
breast tumors and were significantly higher with
increasing tumor grade. An EMT-related expression
profile consisting of decreased membranous and
increased nuclear/cytoplasmic immunoreactivity of Ecadherin and β-catenin and increased expression of Ncadherin, vimentin, Snail, ZEB1 and Twist was observed
in tumor epithelial and stromal component and was
significantly associated with malignant phyllodes breast
tumor histopathology. Interestingly, there was a
significant correlation of ILK expression with all of the
EMT markers examined. Our results suggest that EMT
significantly contributes to phyllodes tumor
pathogenesis and originally implicate ILK and ZEB1 in
phyllodes tumors malignant phenotype.
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