Publication:
Survival of melanopsin expressing retinal ganglion cells long term after optic nerve trauma in mice

Thumbnail Image
Files
Sanchez-Migallon (ExpEyeRes 2018) Survival Mel axotomy long term Mice.pdf(1.74 MB)
Date
2018-09-01
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
María Cielo Sánchez-Migallón ; Francisco J. Valiente-Soriano ; Francisco M. Nadal-Nicolás ; Johnny Di Pierdomenico ; Manuel Vidal-Sanz ; Marta Agudo-Barriuso
item.page.secondaryauthor
Facultades de la UMU::Facultad de Medicina
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
DOI
doi: 10.1016/j.exer.2018.05.029.
item.page.type
info:eu-repo/semantics/article
Description
Abstract
In this study we have compared the response to optic nerve crush (ONC) and to optic nerve transection (ONT) of the general population of retinal ganglion cells in charge of the image-forming visual functions that express Brn3a (Brn3a+RGCs) with that of the sub-population of non-image forming RGCs that express melanopsin (m+RGCs). Intact animals were used as control. ONT and ONC were performed at 0.5 mm from the optic disk, and retinas dissected 3, 5, 7, 14, 30, 45 or 90 days later (n = 5/injury/time point). In all the retinas, Brn3a+RGCs and m+RGCs were identified and their survival analyzed quantitatively and topographically. There were no differences in the course of RGC loss between lesions. The decrease of RGCs was significant at short time points (3 or 5 days for Brn3a+ or m+ RGCs, respectively) and, up to 14 days, the course of loss of both RGC populations was similar, surviving at this time point between 20 and 22% of their original population. However, while the loss of Brn3a+RGCs continues steadily up to 90 days when only 5–6% of them still remain, the loss of m+RGCs stops at 14 days, and the proportion of surviving m+RGCs remains constant up to 90 days (26–30%). In conclusion, m+RGC do not respond to axotomy in the same way than the rest of RGCs, and so whilst imageforming RGCs die in two exponential phases a quick one and a slow protracted one, non-image forming RGCs die only during the first quick phase.
publication.page.subject
Melanopsin , Intrinsically photosensitive RGC , Crush , Transection , Ganglion cells
Citation
Exp Eye Res. 2018 Sep;174:93-97.
URI
http://hdl.handle.net/10201/195369
item.page.embargo
Collections
Artículos
Ir a Estadísticas