Publication: Specific functional features of the cell integrity MAP Kinase pathway in the dimorphic fission yeast Schizosaccharomyces japonicus
Authors
Gómez Gil, Elisa ; Franco Sánchez, Alejandro ; Vázquez Marín, Beatriz ; Prieto Ruiz, Francisco ; Pérez Díaz, Armando Jesús ; Vicente Soler, Jerónima ; Madrid Mateo, María Isabel ; Soto Pino, Teresa ; Cansado Vizoso, José
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Facultades de la UMU::Facultad de Biología
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Publisher
MDPI
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DOI
https://doi.org/10.3390/jof7060482
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info:eu-repo/semantics/article
Description
Abstract
Mitogen activated protein kinase (MAPK) signaling pathways execute essential functions in eu-karyotic organisms by transducing extracellular stimuli into adaptive cellular responses. In the fis-sion yeast model Schizosaccharomyces pombe the cell integrity pathway (CIP) and its core effector, MAPK Pmk1, play a key role during regulation of cell integrity, cytokinesis, and ionic homeostasis. Schizosaccharomyces japonicus, another fission yeast species, shows remarkable differences with re-spect to S. pombe, including a robust yeast to hyphae dimorphism in response to environmental changes. We show that the CIP MAPK module architecture and its upstream regulators, PKC orthologs Pck1 and Pck2, are conserved in both fission yeast species. However, some of S. pombe’s CIP-related functions, such as cytokinetic control and response to glucose availability, are regulated differently in S. japonicus. Moreover, Pck1 and Pck2 antagonistically regulate S. japonicus hyphal differentiation through fine-tuning of Pmk1 activity. Chimeric MAPK-swapping experiments re-vealed that S. japonicus Pmk1 is fully functional in S. pombe, whereas S. pombe Pmk1 shows a limited ability to execute CIP functions and promote S. japonicus mycelial development. Our findings also suggest that a modified N-lobe domain secondary structure within S. japonicus Pmk1 has a major influence on the CIP signaling features of this evolutionarily diverged fission yeast.2021
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Citation
Journal of Fungi, 2021, Vol. 7, Issue, 6 : 482
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