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Type III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems

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dc.contributor.authorSilas, Sukrit
dc.contributor.authorElío Lucas, Patrícia
dc.contributor.authorJackson, Simon A.
dc.contributor.authorAroca Crevillén, Alejandra
dc.contributor.authorHansen, Loren L.
dc.contributor.authorFineran, Peter C.
dc.contributor.authorFire, Andrew Z.
dc.contributor.authorSánchez Amat, Antonio
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2026-01-12T13:00:57Z
dc.date.available2026-01-12T13:00:57Z
dc.date.copyright© Silas et al. 2017
dc.date.issued2017-08-17
dc.description.abstractCRISPR-Cas-mediated defense utilizes information stored as spacers in CRISPR arrays to defend against genetic invaders. We define the mode of target interference and role in antiviral defense for two CRISPR-Cas systems in Marinomonas mediterranea. One system (type I-F) targets DNA. A second system (type III-B) is broadly capable of acquiring spacers in either orientation from RNA and DNA, and exhibits transcription-dependent DNA interference. Examining resistance to phages isolated from Mediterranean seagrass meadows, we found that the type III-B machinery co- opts type I-F CRISPR-RNAs. Sequencing and infectivity assessments of related bacterial and phage strains suggests an ‘arms race’ in which phage escape from the type I-F system can be overcome through use of type I-F spacers by a horizontally-acquired type III-B system. We propose that the phage-host arms race can drive selection for horizontal uptake and maintenance of promiscuous type III interference modules that supplement existing host type I CRISPR-Cas systems.
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dc.format.extent27
dc.identifier.citationeLife, 2017, Vol. 6 : e27601
dc.identifier.doihttps://doi.org/10.7554/eLife.27601.001
dc.identifier.eissn2050-084X
dc.identifier.urihttp://hdl.handle.net/10201/185731
dc.languageeng
dc.publishereLife Sciences Publications
dc.relationNacional: PID2021- 124464NB-I00
dc.relation.publisherversionhttps://elifesciences.org/articles/27601
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleType III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication66095fe4-223f-4dbc-9b73-7cade64d2e20
relation.isAuthorOfPublication15331c24-15e1-4518-824f-57238c36ed42
relation.isAuthorOfPublication.latestForDiscovery66095fe4-223f-4dbc-9b73-7cade64d2e20
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