Publication: Morphological evaluation of steatosis in monolayer cultures (MDCK cells) after treatment with gentamicin and valproic acid
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Date
1989
Authors
Stiemer, B.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
An attempt was made to develop a method
for the morphological and morphometric evaluation of
fat inclusions in monolayer cell cultures. For this purpose
we used electron microscopy (SEM and TEM) and
Sudan black staining methods followed by morphometric
evaluation techniques. MDCK (Madin-Darby-caninekidney)
cells were treated with valproic acid (VPA; 10,
100, 1000 pglml medium for 22 hrs) and gentamicin (1,
10,50 pglml for 60 min). Sudan black staining revealed a
dose-dependent increase in fat deposits after application
of the two substances. SEM inspection showed differences
in the size and distribution of the fat inclusions. TEM
findings only partly allowed us to differentiate clearly
between VPA- and gentamicin-induced fat inclusions.
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