Publication: The inhibitor of calcium activated neutral proteinase is an anti-meiotic agent. The spermicidal and anti-viral action
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Date
1995
Authors
Logothetou-Rella, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In situ cytogenetic morphology and analysis
showed that Epstein-Barr Virus (EBV)-infected Raji and
EBV-producing P3HR-1 cells divide by meiosis and
follow the life cycle of malignant cells in vitro. Meiosis
was documented by the presence of condensed
chromosomes, «o» chromosome, nuclear vlimata (NVs),
NV invasion, extrusion of chromosomes, chromosomal
transfer, metaphase fusion and aneuploidy. EBV-Raji,
EBV-producing P3HR, HIV1-infected MOLT-4 cells
(dividing by meiosis) and human spermatozoa (cellular
products of meiosis) were highly sensitive to the
endogenous inhibitor (CANP-1) of calcium-activated
neutral proteinase (CANP). CANP-1-treated virally
infected and virus producing cells showed necrosis and
disappearance of immunofluorescent viral antigens,
documenting the anti-vira1 action of CANP-1. CANP-Itreated
spermatozoa exhibited arrested motility with
subsequent necrosis documenting the in vitro
spermicidal action of CANP-1. The testis of treated
Wistar rats with 0.25 U CANP-Vgr body weight for six
days, were devoid of spermatozoa, with pronounced
toxicity and exfoliation of spermatocytes and
spermatids, indicating the contraceptive action of
CANP-1 via spermatogenetic arrest. It is concluded that
CANP-1 is an anti-meiotic agent inhibiting CANP
associated with meiosis. Hence CANP-1 is a promising
agent against various diseases involving meiosis.
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