Publication: Sustainable set-ups for the biocatalytic production and scale-up of panthenyl monoacyl esters under solvent-free conditions.
Authors
Nieto Cerón, Susana ; Bernal Palazón, Juana María ; Villa Roca, Rocío ; García-Verdugo, Eduardo ; Donaire González, Antonio ; Lozano Rodríguez, Pedro
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Facultades de la UMU::Facultad de Química
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DOI
10.1021/acssuschemeng.3c00266
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info:eu-repo/semantics/article
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Abstract
A sustainable scaling-up process for the biocatalytic production of new bioactive provitamin-B5 monoacyl esters has been demonstrated. A solvent-free reaction protocol based on the formation of eutectic mixtures between neat substrates render to highly efficient direct esterification of free fatty acids (i.e. from C6 to C18 alkyl chain length) with panthenol catalyzed by lipase. The scale-up from 0.5 to 500 g was evaluated by means of several reaction systems (i.e. ultrasound-assistance, orbital shaking, rotary evaporator, and mechanical stirring coupled to vacuum). For all reactor systems, the yield in panthenyl monoacyl esters was improved by increasing the length of the alkyl chain of the fatty acid (i.e. from 63 % yield for panthenyl butyrate to 83 % yield for panthenyl myristate). The best results (87 % - 95 % product yield, for all cases) were obtained upon a scale-up (50-500 g size) and when a vacuum system was coupled to the biocatalytic reaction unit. Under the optimized conditions a five-fold reduction of the amount of biocatalyst with respect reactors without vacuum was achieved. The recovery and reuse of the immobilized enzyme for 5 operation cycles was also demonstrated. Finally, different metrics have been applied to assess the greenness of the solvent-free biocatalytic synthesis of panthenyl monoesters here reported.
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