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MMP-3 Deficiency Alleviates Endotoxin-Induced Acute Inflammation in the Posterior Eye Segment

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dc.contributor.authorVan Hove, Inge
dc.contributor.authorLefevere, Evy
dc.contributor.authorDe Groef, Lies
dc.contributor.authorSergeys, Jurgen
dc.contributor.authorLibert, Claude
dc.contributor.authorVandenbroucke, Rossmarijn
dc.contributor.authorMoons, Lieve
dc.contributor.authorSalinas Navarro, Manuel Ángel
dc.contributor.departmentAnatomía Humana y Psicobiología
dc.date.accessioned2026-03-05T09:16:41Z
dc.date.available2026-03-05T09:16:41Z
dc.date.copyright© 2016 by the authors
dc.date.issued2016-11-01
dc.description.abstractMatrix metalloproteinase-3 (MMP-3) is known to mediate neuroinflammatory processes by activating microglia, disrupting blood–central nervous system barriers and supporting neutrophil influx into the brain. In addition, the posterior part of the eye, more specifically the retina, the retinal pigment epithelium (RPE) and the blood–retinal barrier, is affected upon neuroinflammation, but a role for MMP-3 during ocular inflammation remains elusive. We investigated whether MMP-3 contributes to acute inflammation in the eye using the endotoxin-induced uveitis (EIU) model. Systemic administration of lipopolysaccharide induced an increase in MMP-3 mRNA and protein expression level in the posterior part of the eye. MMP-3 deficiency or knockdown suppressed retinal leukocyte adhesion and leukocyte infiltration into the vitreous cavity in mice subjected to EIU. Moreover, retinal and RPE mRNA levels of intercellular adhesion molecule 1 (Icam1), interleukin 6 (Il6), cytokine-inducible nitrogen oxide synthase (Nos2) and tumor necrosis factor α (Tnfα), which are key molecules involved in EIU, were clearly reduced in MMP-3 deficient mice. In addition, loss of MMP-3 repressed the upregulation of the chemokines monocyte chemoattractant protein (MCP)-1 and (C-X-C motif) ligand 1 (CXCL1). These findings suggest a contribution of MMP-3 during EIU, and its potential use as a therapeutic drug target in reducing ocular inflammation.
dc.formatapplication/pdf
dc.format.extent23
dc.identifier.citationVan Hove, I., Lefevere, E., De Groef, L., Sergeys, J., Salinas-Navarro, M., Libert, C., ... & Moons, L. (2016). MMP-3 deficiency alleviates endotoxin-induced acute inflammation in the posterior eye segment. International journal of molecular sciences, 17(11), 1825.
dc.identifier.doihttps://doi.org/10.3390/ijms17111825
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10201/218322
dc.languageeng
dc.publisherMDPI
dc.relationThe authors and this work are financially supported by the Hercules Grant (AKUL/09/038 and AKUL/13/09) and by national grants from the Research Council of KU Leuven (KU Leuven BOF-OT/10/033 and BOF-OT/14/064) and the Flemish Institute for the promotion of scientific research (IWT and FWO).
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/17/11/1825
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMMP-3
dc.subjectInflammation
dc.subjectRetina
dc.subjectLPS
dc.subjectBlood retinal barrier
dc.subjectRetinal pigment epithelium
dc.subjectLeukostasis
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleMMP-3 Deficiency Alleviates Endotoxin-Induced Acute Inflammation in the Posterior Eye Segment
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication81da925b-fa46-4a1a-96fd-ce35568fa423
relation.isAuthorOfPublication.latestForDiscovery81da925b-fa46-4a1a-96fd-ce35568fa423
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