Publication: Aerobic training attenuates nicotinic acetylcholine receptor changes in the diaphragm muscle during heart failure
Authors
de Souza, Paula Aiello Tomé ; de Souza, Rodrigo Wagner Alves ; Campos Soares, Luana ; Piedade, Warlen Pereira ; Campos, Dijon Henrique S. ; Carvalho, Robson Francisco ; Padovani, Carlos Roberto ; Okoshi, Katashi ; Cicogna, Antônio Carlos ; Michelin Matheus, Selma Maria ; Dal-Pai-Silva, Maeli
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
10.14670/HH-11-581
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info:eu-repo/semantics/article
Description
Abstract
Introduction: Heart failure (HF) is a
progressive myopathy, with clinical signs of fatigue and
limb weakness that can damage the nerve-muscle
interaction, altering synaptic transmission and nicotinic
acetylcholine receptors (nAChR) in neuromuscular
junctions (NMJs). The diaphragm is composed of a
mixed proportion of muscle fibres, and during HF, this
muscle becomes slower and can alter its function. As
exercise training is an accepted practice to minimise
abnormalities of skeletal muscle during HF, in this study,
we evaluated the hypothesis that aerobic training
attenuates alterations in the expression of nAChR
subunits in NMJs diaphragm during heart failure.
Objective: The aim of this study was to evaluate the
distribution and expression of nAChR subunits in the
diaphragm muscle fibres of rats subjected to an aerobic
training programme during HF. Methods: Control
(Sham), control training (ShamTR), aortic stenosis (AS)
and aortic stenosis training (ASTR) groups were
evaluated. The expression of nAChR subunits (γ, α1, ε,
β1 and δ) was determined by qRT-PCR, and NMJs were
analysed using confocal microscopy. Results: We
observed increased expression of the γ, α1 and β1
subunits in the AS group compared with the ASTR
group. The distribution of NMJs was modulated in these
groups. Discussion: HF alters the mRNA expression of
nAChR subunits and the structural characteristics of
diaphragm NMJs. In addition, aerobic training did notalter NMJs morphology but attenuated the alterations in
heart structure and function and in nAChR subunit
mRNA expression. Our findings demonstrate the
beneficial effects of aerobic exercise training in
maintaining the integrity of the neuromuscular system in
the diaphragm muscle during HF and may be critical for
non-pharmacological therapy to improve the quality of
life for patients with this syndrome.
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Citation
Histology and Histopathology, vol. 30, nº 7, (2015)
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