Publication: Alterations induced on cytoskeleton by Escherichia
coli endotoxin in different types of rat liver cell cultures
Authors
Pagani, R. ; Portolés, M.T. ; De la Viña, S. ; Melzner, I. ; Vergani, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Endotoxins (lipopolysaccharide, LPS) from
Gram-negative bacteria are considered as the agents
responsible for the induction of endotoxic shock,
producing severe cellular metabolic dishomeostasis.
Cytotoxic lesions, as well as functional and metabolic
disturbances, occur mainly in the liver, which is one of
the target organs and exerts an LPS clearance function.
In an attempt to approach the molecular basis of
endotoxic shock, and to propose an experimental model,
we have focused this study on cytoskeleton
(microtubules and microfilaments) alterations induced
by different doses of endotoxin in different target liver
cells. Microfilaments and microtubules were studied by
immunofluorescence and different microscopy
techniques (optic fluorescence microscopy and confocal
laser scanning microscopy) in order to improve the
cytoskeleton study resolution.
Parenchymal and sinusoidal cell morphology,
severely damaged by the LPS action, is related to a
disturbance on the cytoskeletal organisation, even more
evident in a particular proliferating rat liver cell culture.
The most relevant changes are seen in the
microtubule patterns in all liver cells tested, which could
be related, depending on cell type, either to a direct LPS
action or to [Ca+2]i dishomeostasis as well as free radical
and cytokine (IL-1ß and TNF-a) production.
Due to their features, proliferating rat liver cell cultures are used as a sensitive cell model to understand
the effect of LPS on cytoskeleton organisation.
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