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Ocular lesions induced in infant rats by busulfan

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dc.contributor.authorSaito, Tsubasa
dc.contributor.authorAndo, Ryo
dc.contributor.authorOhira, Toko
dc.contributor.authorHoshiya, Toru
dc.contributor.authorTamura, Kazutoshi
dc.date.accessioned2020-03-17T11:55:05Z
dc.date.available2020-03-17T11:55:05Z
dc.date.issued2015
dc.description.abstractAlthough busulfan, a bifunctional alkylating agent, is known to induce cataracts in infant rats, the full nature of busulfan-induced ocular lesions has not yet been shown. In order to clarify this point, 6-day-old rats were treated with a single dose of 20 mg/kg busulfan and the ocular tissue was histopathologically and immunohistochemically examined at 1, 2, 4, 7 and 12 days after treatment (DAT). As a result, in the nuclear layer (NL) of the peripheral retina, apoptotic cells significantly increased at 1 DAT and peaked at 2 DAT when cell proliferating activity was depressed. At 4 DAT, the NL showed wavy deformation with formation of rosette-like structures, and these changes progressed prominently at 12 DAT. In addition, a significant reduction in the retinal thickness due to decreased thickness of NL or inner NL was detected at 2 and 4 DAT. On the other hand, in the germinative zone of the lens equator, apoptotic lens epithelial cells significantly increased from 2 to 7 DAT, resulting in partial loss of lens epithelial cells at 7 and 12 DAT. At 12 DAT, prominent swelling and vacuolation of lens fibers were observed in the area from the equatorial zone to the posterior pole, indicating the development of cataract. The present results strongly suggest that prominent apoptosis in component cells was the initial and essential event underlying the developpment of busulfan-induced ocular lesions in infant rats.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology, Vol. 30, n.º 3 (2015)
dc.identifier.doihttps://doi.org/10.14670/HH-30.321
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/88164
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBusulfanes
dc.subjectInfant rates
dc.subjectOcular lesiones
dc.subjectRosettelike structurees
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleOcular lesions induced in infant rats by busulfanes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30, nº3 (2015)