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Workflow for microbiological diagnosis of bacterial gastroenteritis combining a molecular assay as first-line with reflective stool culture

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dc.contributor.authorMuñoz Dávila, María José
dc.contributor.author Candel-Pérez, C.
dc.contributor.authorVicente, M. R.
dc.contributor.author Piqueras, M.
dc.contributor.author Artero, J.M.
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2025-09-22T10:13:10Z
dc.date.available2025-09-22T10:13:10Z
dc.date.issued2023
dc.description© 2023. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Acta Microbiologica Hellenica.
dc.description.abstractConventional microbiological methods for bacterial enteric disease diagnosis are time-consuming, labour intensive and provide low sensitivity. The aim of this study was to evaluate the results of a new diagnosis strategy which replaces traditional stool culture with a molecular detection using the BD MAX™ System (BD Life Sciences, Sparks, Maryland, United States) as first-line assay together with reflective culture. A total of 1.590 specimens were prospectively requested for stool culture. The molecular detection included the BD MAX enteric bacterial panel together with the BD MAX extended enteric bacterial panel (BDM GIP) performed simultaneously on the same stool specimen. In 18.8% of specimens (176 of the 936 valid samples) there was one or more than one target positive with the following percent positivity: 9.7% Campylobacter spp., 5.7% Salmonella spp., 1.3% Shiga toxin genes (stx1/stx2), 1.2% Shigella spp./enteroinvasive Escherichia coli (EIEC), 1% Yersinia enterocolitica, 1% Vibrio spp. (V. vulnificus/V. parahaemolyticus/V. cholerae), 0.3% Plesiomonas shigelloides, and 0.2% Enterotoxigenic E. coli (ETEC) enterotoxin LT/ST genes. Positive reflective stool culture noted a correlation of 69.5% with the molecular test, missing 23.9% and 15.4% in the cases of Campylobacter spp., and Salmonella spp., respectively. In conclusion, this clinical study demonstrated very good performance of the BDM GIP. The performance and ease of use may provide advantages to many laboratories, improving the detection of bacterial stool pathogens and time to reporting results.
dc.formatapplication/pdf
dc.format.extent11
dc.identifier.citationActa Microbiologica Hellenica, Vol. 68, nº 2, April-June 2023
dc.identifier.eissn2813-9054
dc.identifier.urihttps://hdl.handle.net/10201/159009
dc.languageeng
dc.publisherMDPI
dc.relationSin financiación externa a la Universidad
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectMolecular detection
dc.subjectBD MAX™
dc.subjectSystem
dc.subjectBacterial gastroenteritis
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleWorkflow for microbiological diagnosis of bacterial gastroenteritis combining a molecular assay as first-line with reflective stool culture
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublicatione8d75118-6b22-462a-8382-b884726e369f
relation.isAuthorOfPublication.latestForDiscoverye8d75118-6b22-462a-8382-b884726e369f
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