Publication: Progesterone determination in iberian red deer by time-resolved fluorometry: an alternative method to RIA
Authors
García, Andrés José ; Bernal, Luis Jesús ; Landete-Castillejos, Tomás ; Cerón Madrigal, José Joaquín ; Parra Muñoz, María Dolores
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Publisher
Wiley
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DOI
https://doi.org/10.1002/jez.a.76
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info:eu-repo/semantics/article
Description
© 2004 Wiley-Liss, Inc. This document is the Published version of a Published Work that appeared in final form in Journal of Experimental Zoology Part A: Ecological Genetics and Physiology (JEZ-A). To access the final edited and published work see https://doi.org/10.1002/jez.a.76
Abstract
The validation for Iberian red deer of a commercially available Time-resolved fluoroimmunoassay (TR-FIA) designed for analysis of progesterone in human beings was carried out. Intra-assay coefficients of variation ranged from 3.6% to 7.4%, while inter-assay coefficients of variation varied from 5.2% to 15.5%. Accuracy, evaluated by comparing results yielded by TR-FIA with those obtained from a validated radioimmunoassay (RIA) in the measurement of 14 samples, provided a high regression coefficient (R2 ¼ 0.93). Different progesterone concentrations added to pool plasma showed percentages of recovery that ranged between 102.6% and 82.48%. The limit of detection was 0.102 nmol/L. The results obtained indicate that the present method is suitable for the measurement of progesterone in female Iberian red deer.
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Citation
Journal of experimental Zoology 301A:472–476 (2004)
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